Box-Behnken响应面法优化牡丹皮炭炮制工艺  

Optimization of Processing Technology for Moutan Cortex Carbon by Box-Behnken Response Surface Methodology

作　　者：朱琼花[1] 莫毛燕 孟江[1] 周苏娟[1] 王淑美[1] 梁生旺[1] 

机构地区：[1]广东药学院,广州510006

出　　处：《中国实验方剂学杂志》2015年第14期12-15,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81473352);国家中医药管理局中医药行业科研专项(201207004-7)

摘　　要：目的:优选牡丹皮炭的炮制工艺。方法:采用砂烫法,以5-羟甲基糠醛、没食子酸、儿茶素、丹皮酚含量的综合评分为指标,在单因素试验基础上,选择炮制时间、炮制温度、投料比为自变量,采用Box-Behnken响应面法优化牡丹皮炭的炮制工艺。结果:牡丹皮炭最佳炮制工艺为炮制时间9.0 min,炮制温度180.0℃,投料比25.5倍。5-羟甲基糠醛、没食子酸、儿茶素、丹皮酚质量分数分别为0.022 1%,0.023 3%,0.175 7%,0.042 0%,综合评分预测值97.67分,实测值95.01分。结论:优选的炮制工艺简便可行,预测性良好,充分体现了牡丹皮炭凉血止血、止血而不留瘀的作用特点。Objective： To optimize processing technology of Moutan Cortex carbon. Method： With the content of gallic acid, 5-hydroxymethyl furfural, catechin, paeonol as comprehensive evaluation index, based on single factor tests, herb-to-sand ratio, processing time and temperature were selected as independent variables, sand-scorch method was adopted, processing technology of Moutan Cortex carbon was optimized by Box-Behnken response surface methodology. Result： Optimum processing conditions were as follows： processing time of 9.0 min, processing temperature at 180.0 ~C , herb-to-sand ratio of 25.5： 1. The content of 5-hydroxymethyl furfural, gallic acid, catechin and paeonol were 0. 022 1% , 0. 023 3% , 0. 175 7% and 0. 042 0% , respectively. The measured value of composite score was 95.01, which was close to the predicted value of 97.67. Conclusion： This optimized processing technology of Moutan Cortex carbon is simple and feasible with good predictability, which fully embodies function characteristics of this herb.

关 键 词：牡丹皮炭 5-羟甲基糠醛 没食子酸 儿茶素 丹皮酚 外观性状 

分 类 号：R283.3[医药卫生—中药学] R284.1[医药卫生—中医学]