贵州地方茶树品种资源遗传多样性RAPD分析  

Analysis of Genetic Diversity with RAPD Markers for Local Tea Populations in Guizhou

作　　者：鄢东海 刘声传 罗显扬 魏杰 陆建良[2] 范方媛[2] 

机构地区：[1]贵州省茶叶研究所,贵州湄潭564100 [2]浙江大学茶叶研究所,杭州310058

出　　处：《中国农学通报》2015年第19期30-34,共5页Chinese Agricultural Science Bulletin

基　　金：贵州省创新能力平台建设专项"贵州茶叶种质创新;高效栽培及加工研究创新能力建设"[黔科合院所创新能(2010)4008];贵州省科技重大专项子项目"优良茶树品种选育与适应性栽培技术研究"[黔科合重大专项字(2008)6015–1]

摘　　要：为培育特色优良品种,采用RAPD分子标记对贵州4个地方茶树品种群体的遗传差异进行分析。结果显示,‘贵定鸟王茶’、‘都匀毛尖茶’、‘湄潭苔茶’和‘石阡苔茶’品种群体内种质间遗传距离的变幅较大,分别为0.1792~0.8328、0.1745~0.8518、0.1480~0.6507、0.1516~0.8691,Nei’s基因多样性与Shannon信息指数及品种内RPAD谱带多态性显示它们的遗传多样性依次降低。4个地方群体品种遗传多样性丰富,遗传变异主要发生在群体内。The genetic diversity of populations of four local tea varieties [Camellia sinensis（L.） O.Kuntze] inGuizhou was analyzed using random amplified polymorphic DNA（RAPD） markers to breed cultivars withexcellent characteristics.The genetic distance of four populations including C.sinensis ‘Guiding-niaowangcha’（G）,C.sinensis‘Duyun-maojiancha’（D）,C.sinensis‘Meitan-taicha’（M）,and C.sinensis‘Shiqian-taicha’（S） was 0.1792-0.8328,0.1745-0.8518,0.1480-0.6507,and 0.1516-0.8691,respectively.In addition,based on Nei’s gene diversity index,Shannon’s index,and RAPD bands polymorphism analysis,the genetic diversity among four populations was G〉D〉M〉S.The data showed that these populations had arich genetic diversity.Their genetic variation was mainly distributed within populations.

关 键 词：贵州 地方群体品种 遗传多样性 RAPD标记 

分 类 号：S571.1[农业科学—茶叶生产加工]