代谢综合征患者血清磷脂脂肪酸谱和NF-κB表达的相关性研究  

A correlation study between serum phospholipid fatty acid composition andnuclear factor kappa-light-chain-enhancer of activated B cellsin patients with metabolic syndrome

作　　者：黄迪华[1] 楼大钧[1] 朱麒钱[1] 董海燕[1] 李铎[2] 任跃忠[3] 

机构地区：[1]浙江省绍兴市人民医院(浙江大学绍兴医院)内分泌代谢科,312000 [2]浙江大学食品科学与营养系,杭州市310029 [3]浙江大学医学院附属第二医院内分泌代谢科,杭州市310009

出　　处：《中华全科医学》2015年第9期1435-1436,1464,共3页Chinese Journal of General Practice

基　　金：中华医学会临床医学科研专项资金支持项目(13050750460);浙江省绍兴市科技局重点资助项目(2013B70074)

摘　　要：目的研究代谢综合征(MS)患者血清磷脂脂肪酸谱与核因子-κB(NF-κB)表达的相关性及其意义。方法以60例MS患者和60名健康对照(NC)者为研究对象,采用ELISA法测定血清中NF-κB的表达,高效气相色谱法测定血清磷脂脂肪酸谱。采用t检验进行组间比较分析,用Pearson相关性分析来分析血清磷脂脂肪酸谱和NF-κB的相关性。结果 MS组NF-κB表达为(11.66±3.63)ng/ml,明显高于NC组(P<0.01)。MS组的饱和脂肪酸(SFA)含量为(41.59±3.51)%,较NC组显著升高(P<0.01),多不饱和脂肪酸(PUFA)含量为(47.17±2.51)%,较NC组显著降低(P<0.01),2组之间的单不饱和脂肪酸(MUFA)无统计学差异。Pearson相关性分析显示,MS组中NF-κB的表达与SFA呈显著正相关(r=0.42,P=0.00),与n-6 PUFA、n-3 PUFA呈显著负相关(r=-0.33,-0.19,P=0.00,0.03)。结论 MS患者的血清磷脂脂肪酸构成中SFA比例增加,PUFA比例减少,且与NF-κB表达增加有关,提示膳食结构中SFA增多、PUFA减少可能通过NF-κB通路的激活而引起或加重MS的炎症状态。Objective To investigate the relevance and its significance between nuclear factor kappa-light-chain-enhancer of activated B cells（NF-KB） and serum phospholipid fatty acid composition in patients with metabolic syndrome （MS）. Methods Sixty eases of MSpatientsand 60 normalsubjects （controlgroup） were enrolled in the study. ELISA was used to measure the express of NF-KB in serum, and gaschrematography-mass spectrometer was employed to test serum phospholipid fatty acid profile. T test was used to the comparative analysis between the two groups, and Pearson correlation analysis was used to analyze the correlation between serum phospholipid fatty acid and NF-KB. Results The expression level of NF-KB in MS group was （ 11.66 ± 3.63 ） ng/rnl, significantly higher than that in the control group （ P 〈0. 01 ）. The percentage of saturated fatty acid（SFA） were （41.59 ± 3.51 ） % ,higher than those in control group. The percentage of poly- unsaturated fatty acid（PUFA） were （47. 17± 2. 51 ）%, lower than those in control group. The percentage of monounsat- urated fatty acids（MUFA） between the two groups didn＇t have the statistical difference. The Pearson＇ s correlation analy- sis showed that the expression level of NF-KB was positively correlated with SFA （ r = 0. 42, P = 0. 003 ）, but negatively correlated with n-6 PUFA and n-3PUFA（r = -0. 33, -0. 19,P =0. 00,0. 03,respectively）. Conclusion The MS patients had increasedproportionofSFA and reduced proportionofPUFA. These changes in serumphospholipidfattyacidcompositions were correlatedwiththeincreasedexpressionofNF-kB,which suggest that the increased intake of SFA and reduced intake of PUFA in the dietary pattern maybe caused or aggravated the inflammatory state of MS through the overexpression of the NF-κB pathway.

关 键 词：代谢综合征 NF-ΚB 血清磷脂脂肪酸 饱和脂肪酸 多不饱和脂肪酸 

分 类 号：R589[医药卫生—内分泌]