真海鞘壳化学成分分离及其浸膏抑制人肝癌细胞HepG2活性的研究  

Isolation of constituents in Halocynthia roretzi Drasche sell and inhibitory activity of extracts from H.roretzi against HepG2 cells

作　　者：翟兴月[1] 侯秀秀[2] 佟长青[2] 杜影[3] 李伟[2] 

机构地区：[1]大连医科大学附属第二医院临床营养科,辽宁大连116023 [2]大连海洋大学食品科学与工程学院,辽宁大连116023 [3]大连市疾病预防控制中心,辽宁大连116021

出　　处：《河北渔业》2015年第7期16-19,共4页

基　　金：海洋局公益性行业科研专项(项目编号201205022-7)

摘　　要：采用硅胶柱色谱及薄层制备色谱等手段从真海鞘(Halocynthia roretzi Drasche)壳中分离纯化出了3种化合物。对所获得的化合物经过理化性质、波谱分析和文献对照的方法进行了结构鉴定。同时,采用CCK-8方法对75%EtOH浸提所获得的真海鞘壳浸膏进行了抑制人肝癌细胞HepG2生长测试。结果表明,从真海鞘壳中分离得到3种化合物分别为:Diethyl 24-[(Z)-3-methy-1-hexenyl]-heptatetracontanedioate(化合物1)、胆甾醇(化合物2)和6-(2,,3,-Dihydroxy-4,-hydroxymethyl-tetrahydro-furan-1,-yl)cyclopentadiene[c]pyrrole-1,3-diol(化合物3)。体外测试表明,75%EtOH浸提所获得的真海鞘壳浸膏具有显著抑制人肝癌细胞HepG2生长的活性,可作为潜在的抗肿瘤药物进行进一步研究。Three compounds were isolated from Halocynthia roretzi Drasche shell by column chromatography on silica gel and thin layer chromatography.Their structures were identified by spectroscopic analysis and physical-chemistry properties.The inhibition on HepG2 cells of all extracts from H.roretzi sell were evaluated by CCK-8assay.The results showed that three compounds were obtained and identified as Diethyl 24-[（Z）-3-methy-1-hexenyl]-heptatetracontanedioate（compound1）,cholesterol（compound 2）and 6-（2＇,3＇-dihydroxy-4＇-hydroxymethyl-tetrahydro-furan-1＇-yl）cyclopentadiene[c]pyrrole-1,3-diol（compound 3）.All extracts from H.roretzi sell using75% EtOH exhibited the potent inhibitory activities against HepG2 cells.The extracts could be considered as potential anticancer candidate for further study.

关 键 词：真海鞘 化学成分 结构鉴定 人肝癌细胞HEPG2 

分 类 号：R735.7[医药卫生—肿瘤]