丹参酮ⅡA对心肺复苏大鼠脑水通道蛋白4表达及脑水肿的影响  

Influence of tanshinone Ⅱ A on expression of aquaporin-4 and cerebral edema after cardiopulmonary resuscitationin rats

作　　者：张洁[1] 罗序睿[2] 陈耿靖[2] 张慧利[2] 

机构地区：[1]南昌大学第一附属医院急诊药房,330006 [2]南昌大学第一附属医院急诊科,330006

出　　处：《中国医药》2015年第8期1133-1136,共4页China Medicine

基　　金：江西省卫生厅中医药科研基金（2010A074）

摘　　要：目的 探讨丹参酮ⅡA对心肺复苏大鼠脑水通道蛋白4（AQP4）表达及脑水肿的影响.方法 将78只SD雄性大鼠随机分为3组：假手术组（6只）,对照组（36只）,丹参酮ⅡA干预组（36只）,采用窒息致大鼠心脏骤停动物模型,丹参酮ⅡA干预组复苏即刻予以丹参酮ⅡA 15 mg/kg静脉推注,对照组予以等量0.9％氯化钠注射液静脉推注,在自主循环恢复后（ROSC）1、6、12、24、48、72 h断头取脑,应用免疫组织化学染色法检测脑组织AQP4表达,干湿重法测量脑组织含水量.结果 对照组在ROSC后1、6、12、24、48、72 h各时点脑AQP4蛋白的表达分别为10.2±1.9、13.4 ±2.1、15.1±2.0、20.5±3.0、17.6±2.9、14.5±2.2,均高于假手术组的7.3±1.2,差异均有统计学意义（均P＜0.01）;丹参酮ⅡA干预组在ROSC后1、6、12、24、48、72 h各时点脑AQP4的表达分别为9.8±1.2、11.0±1.9、14.3±2.2、17.1±2.5、16.1±1.7、13.6±2.2,均低于对照组同时点,差异均有统计学意义（P＜0.01或P＜0.05）.对照组在ROSC后1、6、12、24、48、72 h各时点脑组织含水量分别为（78.22±0.23）％、（78.39±0.16）％、（79.11±0.24）％、（79.43 ±0.20）％、（78.67±0.21）％、（78.06±0.20）％,均高于假手术组的（77.28±0.09）％,差异均有统计学意义（均P＜0.01）;丹参酮ⅡA干预组在ROSC后1、6、12、24、48、72 h各时点脑组织含水量分别为（77.60±0.16）％、（77.96±0.21）％、（78.17±0.21）％、（78.77±0.28）％、（78.05±0.23）％、（77.61±0.23）％,均低于对照组同时点,差异均有统计学意义（P＜0.01或P＜0.05）.结论 丹参酮ⅡA能降低大鼠心肺复苏后脑组织AQP4的表达,减轻脑水肿.Objective To investigate the influence of tanshinone Ⅱ A on the expression of aquaporin-4 （AQP-4） and cerebral edema after cardiopulmonary resuscitationin rats.Methods Totally 78 Sprague Dawley male rats were randomly divided into sham group（n =6）,control group （n =36） and tanshinone Ⅱ A intervention group （n =36）.Cardiac arrest was induced by choking models in control group （n =36） and tanshinone Ⅱ A intervention group;tanshinone Ⅱ A intervention group received intravenous injection of tanshinone Ⅱ A （15 mg/kg） and control group was injected with the same amount of 0.9% sodium chloride injection at recovery instantly.The expression of AQP4 in brain tissue was measured by Immune-histochemical staining at 1,6,12,24,48 and 72 h after restoration of spontaneous circulation （ROSC）;the water content of the brain was measured by wet/dry weight method.Results The expression of AQP-4 in control group at 1,6,12,24,48,72 h after ROSC was 10.2 ± 1.9,13.4 ±2.1,15.1 ±2.0,20.5 ±3.0,17.6 ±2.9,14.5 ±2.2,respectively,all significantly higher than that in sham group （7.3 ± 1.2） （P 〈 0.01）;the expression of AQP-4 in tanshinone Ⅱ A intervention group at all time points was 9.8 ± 1.2,11.0 ± 1.9,14.3 ± 2.2,17.1 ± 2.5,16.1 ± 1.7,13.6 ± 2.2 respectively,all significantly lower than that in control group （P 〈 0.01 or P 〈 0.05）.The water content of brain tissue in control group at 1,6,12,24,48,72 h after ROSC was （78.22 ±0.23）%,（78.39 ± 0.16）%,（79.11 ±0.24）%,（79.43 ±0.20）%,（78.67 ±0.21）%,（78.06 ±0.20）%,respectively,all significantly higher than that in sham group [（77.28 ± 0.09）%] （P 〈 0.01）;the water content of brain tissue intanshinone Ⅱ A intervention group was （77.60 ±0.16）%,（77.96 ±0.21）%,（78.17 ±0.21）%,（78.77 ± 0.28） %,（78.05 ± 0.23） %,（77.61 ± 0.23） %,respectively,all significantly lower than that in control group （P 〈 0.01 or P 〈 0.05）.Conclusion Tanshinone Ⅱ A exert can reduce t

关 键 词：脑水肿 丹参酮ⅡA 水通道蛋白质4 心搏骤停 心肺复苏 

分 类 号：R742.7[医药卫生—神经病学与精神病学]