Selective silencing of 2Cys and type-ⅡB Peroxiredoxins discloses their roles in cell redox state and stress signaling  

作　　者：Patrícia Vidigal Ana Montserrat Martin-Hernandez Cèlia Guiu-Aragonés Sara Amncio Luísa Carvalho 

机构地区：[1]Departamento de Recursos Naturais,Ambiente e Território (DRAT)/Centro de Botanica Aplicada à Agricultura (CBAA),Institute of Agronomy,University of Lisbon [2]Institut de Recercai Tecnologia Agroalimentàries (IRTA),Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB) Campus Universitat Autonoma de Barcelona (UAB),Edificio Center for Research in Agricultural Genomics (CRAG)

出　　处：《Journal of Integrative Plant Biology》2015年第6期591-601,共11页植物学报（英文版）

基　　金：funded by Fundao para a Ciência e Tecnologia (FCT): CBAA (Pest OE/AGR/UI0240/2011);the postdoc grants SFRH/BPD/43898/2008 to PV;SFRH/BPD/85767/ 2012 to LC

摘　　要：Peroxiredoxins （Prx） catalyse the reduction of hydrogen peroxide （H2O2） and, in association with catalases and other peroxidases, may participate in signal transduction by regulating intercel ular H2O2 concentration that in turn can control gene transcription and cel signaling. Using virus-induced-gene-silencing （VIGS）, 2-Cys Peroxiredoxin （2CysPrx） family and type-II Peroxiredoxin B （PrxI B） gene were silenced in Nicotiana benthamiana, to study the impact that the loss of function of each Prx would have in the antioxidant system under control （22℃） and severe heat stress conditions （48 ℃）. The results showed that both Prxs, although in different organel es, influence the regeneration of ascorbate to a significant extent, but with different purposes. 2CysPrx affects abscisic acid （ABA） biosynthesis through ascorbate, while PrxIIB does it probably;through the xanthophyl cycle. Moreover, 2CysPrx is key in H2O2 scavenging and in consequence in the regulation of ABA signal-ing downstream of reactive oxygen species and PrxIIB provides an important assistance for H2O2 peroxisome scavenges.Peroxiredoxins （Prx） catalyse the reduction of hydrogen peroxide （H2O2） and, in association with catalases and other peroxidases, may participate in signal transduction by regulating intercel ular H2O2 concentration that in turn can control gene transcription and cel signaling. Using virus-induced-gene-silencing （VIGS）, 2-Cys Peroxiredoxin （2CysPrx） family and type-II Peroxiredoxin B （PrxI B） gene were silenced in Nicotiana benthamiana, to study the impact that the loss of function of each Prx would have in the antioxidant system under control （22℃） and severe heat stress conditions （48 ℃）. The results showed that both Prxs, although in different organel es, influence the regeneration of ascorbate to a significant extent, but with different purposes. 2CysPrx affects abscisic acid （ABA） biosynthesis through ascorbate, while PrxIIB does it probably;through the xanthophyl cycle. Moreover, 2CysPrx is key in H2O2 scavenging and in consequence in the regulation of ABA signal-ing downstream of reactive oxygen species and PrxIIB provides an important assistance for H2O2 peroxisome scavenges.

关 键 词：2-Cys Peroxiredoxin abscisic acid ASCORBATE heat stress hydrogen peroxide PEROXISOME type-Ⅱ Peroxiredoxin B 

分 类 号：Q942[生物学—植物学]