NPTX2基因甲基化与年龄推断的相关性研究  

Correlation between the methylation of NPTX2 gene and age estimation

作　　者：邢扬[1] 幸宇[1] 李倩楠[1] 甘丽[1] 邓世雄[1] 

机构地区：[1]重庆医科大学基础医学院法医学教研室,重庆400016

出　　处：《重庆医科大学学报》2015年第4期594-598,共5页Journal of Chongqing Medical University

基　　金：国家自然科学基金青年科学基金资助项目(编号:81202385)

摘　　要：目的:探讨神经元正五聚蛋白2(neuronal pentraxinⅡ,NPTX2)基因启动子区Cp G位点甲基化与年龄的关系。方法:提取170例活体外周血样本的基因组DNA,经甲基化修饰试剂盒修饰后,利用甲基化特异性-PCR(methylation specific-PCR,MSP)检测其NPTX2基因启动子区甲基化情况,并分析其与性别、年龄的关系。结果:以每个样本年龄、性别为自变量,是否甲基化为因变量,用二分类的logistic回归分析显示,是否甲基化与性别因素不具有统计学意义(P=0.843),表明性别因素对NPTX2基因甲基化影响不明显;而是否甲基化与年龄因素则具有统计学意义(P=0.000),表明年龄因素显著影响该基因的甲基化。以五岁为一个年龄段,对其定性情况进行线性回归分析显示男性(P=0.001)、女性(P=0.000)、总体(P=0.000)均存在线性关系,表明甲基化率是随年龄段的增加而呈线性增长的。结论:NPTX2基因启动子区甲基化程度随年龄的增加而增加。可进一步研究建立回归模型,进行年龄推断。Objective：To investigate the relationship between age and the methylation of Cp G loci,which is in the neuronal pentraxinⅡ（NPTX2）gene＇s promoter region. Methods：Genomic DNA was extracted from 170 cases of peripheral blood samples of living,and then modified by methylation modification kit. The NPTX2 gene promoter methylation status was detected by the methylation specific-PCR（MSP）method,and its relationship with gender,age was analyzed. Results：Age and sex of each sample were taken as independent variables while whether having methylation was taken as dependent variable. Binary logistic regression analysis showed that statistical significance was not found between whether having methylation and gender factor（P =0.843）,suggesting that gender does not affect NPTX2 gene methylation;and statistical significances were found between whether having methylation and age factor（P=0.000）,suggesting that age factors significantly influences the gene methylation. Taking five-year-old as an age group,linear regression analysis revealed that there were linear relationship among male（P=0.001）,female（P=0.000）and the overall（P=0.000）,indicating that methylation rate is linearly increased with the increase of age. Conclusion：The methylation rate of NPTX2 gene promoter increased with age. Further research may establish a regression model for age estimation.

关 键 词：神经元正五聚蛋白2 年龄推断 甲基化 甲基化特异性-PCR 

分 类 号：R734.2[医药卫生—肿瘤]