不同方法对食源性致病菌耐药性监测研究  

Comparison of antimicrobial resistance surveillance of foodborne pathogens by different methods

作　　者：沈赟[1] 秦思[1] 马恺[1] 唐震[1] 

机构地区：[1]江苏省疾病预防控制中心,南京210009

出　　处：《江苏预防医学》2015年第4期9-11,共3页Jiangsu Journal of Preventive Medicine

摘　　要：目的了解江苏地区食源性致病菌的常用抗菌药耐药谱,评价不同药敏方法在致病菌耐药性监测中的适用性。方法用Etest和微量肉汤稀释法,对江苏省2012-2014年食源性疾病常规监测检出的金黄色葡萄球菌、副溶血性弧菌、沙门菌菌株进行药敏鉴定。结果金黄色葡萄球菌对红霉素的耐药率最高,其次为复方磺胺甲噁唑和四环素,且有多重耐药;副溶血性弧菌除对四环素和复方磺胺甲噁唑有一定的耐药,对其他6种革兰氏阴性菌抗生素均敏感。沙门菌对萘啶酸的耐药率超过50.0%。两种方法药敏检测结果对不同抗生素符合率有差异,但符合率均>80%。结论 Etest和微量肉汤稀释法有较好的一致性,但所需时间及操作要求有差异,可根据实际需要进行选择。Objective To investigate common antimicrobials spectrum of foodborne pathogens isolated in Jiangsu Province; to evaluate performance of different methods in foodborne pathogen antimicrobial resistance surveillance. Methods Etest and microscale broth dilution method were used for antimicrobial susceptibility test of Staphylococcus aureus, Vibrio Parahemo- lyticus and Salmonella isolates identified in routine foodborne pathogen surveillance in Jiangsu Province from 2012 to 2014. Results Staphylococcus aureus isolates had the highest resistance to erythromycin, followed by compound sulfamethoxazole and tetracycline. Some Staphylococcus aureus isolates were resistant to multiple antimicrobials. Vibrio Parahemolyticus were resistant to tetracycline and compound sulfamethoxazole at certain levels, and sensitive to 6 antlmicroblals against Gram nega- tive bacteria. More than 50% of Salmonella isolates were resistant to nalidixie acid. Drug resistance rates obtained via 2 meth- ods were slightly different,with consistence of more than 80%. Conclusion Etest and microscale broth dilution method were generally consistent in antimierobial susceptibility test, proper method should be selected according to actual time and operating requirements.

关 键 词：食源性致病菌 ETEST 微量肉汤烯释法 抗菌药物 耐药性 

分 类 号：TS201.3[轻工技术与工程—食品科学]