螺旋藻蛋白酶解剂的筛选与优化  

Screening and Optimization of Hydrolysis of Spirulina Proteins by Enzymes

作　　者：张军[1] 邹宁[1] 孙东红[1] 曲青梅 李小慧[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264000

出　　处：《安徽农业科学》2015年第22期242-244,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]筛选并优化螺旋藻蛋白酶解剂。[方法]采用酶解法初步研究了中性蛋白酶、复合蛋白酶、风味蛋白酶、碱性蛋白酶4种蛋白酶对螺旋藻蛋白质的水解效果,同时研究了底物浓度和酶的添加量对水解率的影响。[结果]结果表明,蛋白酶按酶解螺旋藻蛋白质的水解率,可由高到低依次排列为:碱性蛋白酶、风味蛋白酶、复合蛋白酶、中性蛋白酶。通过对酶解条件进行优化,发现碱性蛋白酶的作用效果受p H影响最大,最高水解率为60.5%(底物浓度20 g/L、E/S=4%、p H=8.9、55℃、酶解5 h)。同时得出,碱性蛋白酶、复合蛋白酶、中性蛋白酶的最佳底物浓度均为20 g/L,最佳酶添加量均为4%,而风味蛋白酶的最佳底物浓度为20 g/L,最佳酶添加量为2%。[结论]研究可为后续功能性多肽的提取利用提供参考依据。[Objective] To screen and optimize Spirulina proteinase solution. [Method] Hydrolysis efficiency of four kinds of enzymes（ neutral protease,complex protease,flavor protease,alkaline protease） on Spirulina proteins were preliminarily studied in this work. The effects of substrate concentration and enzyme adding amount on hydrolysis rate were studied. [Result]The results showed that the hydrolysis of alkali protease had the maximal HE which was followed by the efficiency of flavor enzyme,compound proteinase,neutral protease,sequentially. The efficiency of the alkali protease was apparently affected by pH compared with the others. Its maximal HE was 60. 5%（ substrate 20 g / L,E / S = 4%,pH =8. 9,55 ℃,hydrolysis 5 h）. The ratios of enzyme to substrate（ E / S） and the substrate concentrations were optimized as well. The optimal substrate concentrations for the three kinds of enzymes was 20 g / L. The optimal enzyme concentrations was 4% for them. Whileoptimal flavor enzyme concentration was 2%,and its optimal substrate concentration was 20 g / L. [Conclusion]The study can provide reference basis for extraction and utilization of follow-up functional peptide.

关 键 词：螺旋藻 蛋白质 酶解 水解率 优化 

分 类 号：S917[农业科学—水产科学] Q7[生物学—分子生物学]