克罗诺杆菌分子鉴定方法的比较  

Comparison on molecular identification methods of Cronobacter spp.

作　　者：迟涛[1] 方景泉[1] 满朝新[1] 许慧[2] 

机构地区：[1]国家乳业工程技术研究中心黑龙江省乳品工业技术开发中心东北农业大学,哈尔滨150028 [2]国家大豆工程技术研究中心黑龙江省大豆技术开发研究中心东北农业大学,哈尔滨150028

出　　处：《中国乳品工业》2015年第7期10-12,共3页China Dairy Industry

基　　金："十二五"国家科技支撑计划项目(2013BAD18B11)

摘　　要：克罗诺杆菌(原名阪崎肠杆菌)是能够感染婴儿和成人的机会性致病菌,尤其是能够通过污染婴儿配方乳粉感染新生儿,严重威胁婴幼儿的健康。选取11株分离自婴儿配方乳粉中的克罗诺杆菌为研究对象,利用16S r RNA和看家基因fus A对克罗诺杆菌进行分子层面的鉴定,并得到了两者清晰的系统发育关系,结果表明两种分子鉴定的方法都能对克罗诺杆菌进行鉴定,且基于看家基因fus A的鉴定方法具有更高的辨识度,能将克罗诺杆菌的鉴定精确到种。因此利用看家基因fus A对克罗诺杆菌进行分子鉴定具有优越性,本研究结果有助于克罗诺杆菌的分子鉴定,为克罗诺杆菌的进一步研究提供了理论基础。Cronobacter spp. （Enterobacter sakazaki） are opportunistic bacterial pathogens of both infants and adults. More importantly for neonatal health, it can cause severe neonatal infections through the ingestion of contaminated PIF. In our study, 11 Cronobacter strains isolated from PIF were identified by 16S rRNA and fusA gene. The clear phylogenetic relationships of Cronobacter were obtained. The results showed that Cronobacter spp. can be identified using two molecular identification methods, and the identification method based on housekeeping gene fusA had higher discrimination, can identify Cronobacter strians at the level of species. Therefore, the molecular identification method based on housekeeping gene fusA was superior. This study can be helpful to the molecular identification of Cronobacter spp., provides a theoretical basis for further research on Cronobacter spp..

关 键 词：克罗诺杆菌 分子鉴定 16S RRNA fusA 

分 类 号：Q93-331[生物学—微生物学]