茯苓多糖对糖尿病小鼠淋巴细胞DNA损伤及肾组织主要抗氧化酶活性的影响  

The effect of pachymaran on the lymphocyte DNA damage and the activity of main antioxidant enzymes in the kidney tissues of diabetic mice

作　　者：黄聪亮[1,2] 郑佳俐[1,2] 李凤林[3] 宫敬利[3] 

机构地区：[1]漳州职业技术学院食品与生物工程系,福建漳州363000 [2]农产品深加工及安全福建省高校应用技术工程中心,福建漳州363000 [3]吉林农业科技学院食品工程学院,吉林吉林132101

出　　处：《黑龙江畜牧兽医》2015年第7期35-37,42,286,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：吉林省科技发展计划重点支撑项目"天然植物多糖提取工艺及产品开发的研究"(20090905)

摘　　要：为研究茯苓多糖对2型糖尿病(NIDDM)小鼠淋巴细胞DNA损伤及肾组织主要抗氧化酶活性的影响,试验采用高糖高脂饲料+小剂量链脲佐菌素(STZ)方式诱导NIDDM动物模型,然后将动物分成正常对照组、模型对照组、茯苓多糖灌胃组、罗格列酮灌胃组,药物连续灌胃42 d后,检测小鼠淋巴细胞DNA损伤,肾组织超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性以及丙二醛(MDA)含量。结果表明:WRP对NIDDM小鼠淋巴细胞DNA损伤具有修复作用,能提高糖尿病小鼠肾组织中SOD、GSH-Px、CAT活性,降低MDA的含量。表明茯苓多糖能增强肾脏抗氧化性,保护自由基介导的氧化损伤,可以治疗或延缓糖尿病肾病(DN)的发生。To study the effect of pachymaran on lymphocyte DNA damage and the activity of main antioxidant enzymes in the kidney tissues of non- insulin- dependent diabetes mellitus（ NIDDM） mice,the high- sugar and high- fat feed plus low- dose streptozotocin（ STZ） were used to induce the animal model of NIDDM. And then the mice were randomly divided into five groups,including normal control group,model control group,pachymaran group by gavage,and rosiglitazone group by gavage. The lymphocyte DNA damage,the activity of superoxide dismutase（ SOD）,glutathione peroxidase（ GSH- Px）,catalase（ CAT）,and the content of malondialdehyde（ MDA） in the kidney tissues of the mice were measured after 42 days of treatment with the drugs. The result showed that WRP could restore lymphocyte DNA damage,and enhance the activity of SOD,GSH- Px and CAT,and decrease the level of MDA in the kidney tissues of diabetic mice. The results indicate that pachymaran can enhance the renal antioxidant activity and protection against free radical- mediated oxidative damage,which can treat diabetic nephropathy（ DN） or delay its occurrence.

关 键 词：茯苓多糖 糖尿病 淋巴细胞 肾组织 DNA损伤 抗氧化酶 

分 类 号：S853.76[农业科学—临床兽医学] S854.43[农业科学—兽医学]