冷冻结晶法富集藻油中DPA和DHA甘油酯的研究  

Enrichment of DPA and DHA glycerides from algae oil by freezing crystallization

作　　者：王靖[1] 胡志雄[1,2] 许春芳[1] 齐玉堂[1] 张维农[1,2] 

机构地区：[1]武汉轻工大学食品科学与工程学院,武汉430023 [2]农产品加工湖北省协同创新中心,武汉430023

出　　处：《中国油脂》2015年第7期69-72,共4页China Oils and Fats

基　　金：国家粮食公益性行业科研专项(201313007);武汉轻工大学食品营养与安全研究院重大项目培育专项(2011z02)

摘　　要：以冷冻结晶法对藻油中二十二碳五烯酸(DPA)和二十二碳六烯酸(DHA)甘油酯进行了分离富集研究,并采用气相色谱-质谱联用对产物中脂肪酸组成及含量进行分析。实验确定的最佳工艺条件为:以丙酮为溶剂,藻油与溶剂体积比1∶6,结晶温度-40℃,结晶时间8 h。在最佳条件下,富集藻油中DPA含量从11.65%提升至14.36%,DHA含量从44.64%提升至57.47%。The enrichment of docosapentenoic acid （DPA） and docosahexaenoic acid （DHA） glycerides from algae oil by freezing crystallization was studied. The fatty acid composition uct were determined by GC -MS. The optimal process conditions were obtained and content of the prod- as follows： with acetone as solvent, volume ratio of algae oil to solvent 1 ： 6, crystallization temperature - 40 ℃, crystallization time 8 h. Under the optimal conditions, the content of DPA in enriched algae oil increased from 11.65 % to 14.36% and the content of DHA improved from 44.64% to 57.47%.

关 键 词：藻油 DHA DPA 甘油酯 冷冻结晶 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ028[轻工技术与工程—食品科学与工程]