浸润T淋巴细胞在肿瘤微环境中诱导髓系来源的抑制性细胞募集的实验研究  

Infiltrated T Cells Induce the Accumulation of Myeloid-derived Suppressor Cells in Tumor Microenvironment

作　　者：柳晓芸 刘妍彤[2] 毛野[2] 杜晶[2] 杜薇[2] 余佳耘 罗敏[2] 桑亚雄 高祥[2] 徐广超[2] 刘肖肖[2] 邵彬[2] 魏于全[2] 

机构地区：[1]四川大学生命科学学院,成都610065 [2]四川大学华西医院生物治疗国家重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2015年第4期542-547,共6页Journal of Sichuan University(Medical Sciences)

摘　　要：目的通过研究浸润T淋巴细胞的存在对髓系来源的抑制性细胞(MDSC)数量变化的影响,探讨浸润T淋巴细胞在肿瘤微环境中对MDSC的募集作用。方法建立BALB/c小鼠和免疫缺陷裸鼠荷瘤模型,进行裸鼠荷瘤T淋巴细胞过继实验和抗体阻断T淋巴细胞实验,观察对肿瘤生长的影响,采用肿瘤组织HE染色观察病理组织学变化,采用流式细胞术(FACS)检测肿瘤组织内MDSC的数量变化。结果荷瘤裸鼠肿瘤较正常小鼠荷瘤的肿瘤生长更快(P<0.05),肿瘤微环境中MDSC的百分率更低,与正常小鼠肿瘤组织相比差异有统计学意义(P<0.05)。接受T淋巴细胞过继后的荷瘤裸鼠肿瘤微环境中MDSC百分率有增加(P<0.05)。接受抗体阻断实验的过继荷瘤裸鼠肿瘤组织内MDSC百分率减少(P<0.05)。结论肿瘤微环境中浸润T淋巴细胞能直接诱导MDSC的募集,并对肿瘤生长产生影响。Objective To determine the role of infiltrated T cells in the accumulation of myeloid-derived suppressor cells（MDSCs）in tumor microenvironment. Methods T-cell-deficient nude mice models were established using BALB/c mice.Growth of tumors was compared between those with and without adoptive transfer of T cells.Pathological changes of the tumors were examined with HE histological analysis.The levels of MDSCs were detected with flow cytometry（FACS）.Results Tumor growth was promoted in T-cell-deficient nude mice,which was accompanied with lower levels of MDSCs compared with BALB/c mice（P〈0.05）.T cell transfer increased the level of MDSCs significantly（P〈0.05）.T cells depletion decreased the level of MDSCs（P〈0.05）.Conclusion Infiltrated T cells induce the accumulation of MDSCs in tumor microenvironment,and influence tumor growth.

关 键 词：肿瘤微环境 浸润T淋巴细胞 髓系抑制性细胞 

分 类 号：R730.3[医药卫生—肿瘤]