不同碳源和氮源对金针菇降解木质纤维素酶活性的影响  

Effects of carbon and nitrogen sources on lignocellulose decomposition enzyme activities in Flammulina velutipes

作　　者：安琪[1] 吴雪君[1] 吴冰[1] 戴玉成[1] 

机构地区：[1]北京林业大学微生物研究所,北京100083

出　　处：《菌物学报》2015年第4期761-771,共11页Mycosystema

基　　金：国家重点基础研究发展计划(973计划)(2014CB138301)

摘　　要：以3株栽培的金针菇Flammulina velutipes为材料,研究它们在玉米芯和棉子壳以及不同碳源、氮源培养条件下纤维素、半纤维素和木质素降解酶活性的规律。结果表明,不同金针菇菌株的羧甲基纤维素酶、木聚糖酶和漆酶活力显著不同(P<0.001),同时,培养条件对羧甲基纤维素酶、木聚糖酶和漆酶的活力都有显著影响(P<0.001)。在简单碳源存在的条件下,金针菇的羧甲基纤维素酶和木聚糖酶活力远远低于复杂碳源培养基(P<0.05)。全营养培养基上生长的金针菇的羧甲基纤维素酶和木聚糖酶活力低于缺乏碳源和氮源的培养基(P<0.05)。漆酶活力在无简单氮源培养基上低于全培养基(P<0.05)和无葡萄糖培养基(P<0.05),即复杂碳源和氮源培养基上的漆酶活力低于简单碳源和氮源培养基(P<0.05)。Three Flammulina velutipes strains were used to study the effects of corncob and cottonseed hull as well as different carbon and nitrogen sources on cellulose, hemicellulose and lignin decomposition enzyme activities. The activities of CMCase, xylanase and laccase indicated significant differences among Flammulina velutipes strains （P〈0.001）, and cultural media significantly affected the activities of CMCase, xylanase and laccase in Flammulina velutipes （P〈0.001）. The CMCase and xylanase activities on medium with simple carbon sources were significantly reduced than those on medium with complicated carbon sources （P〈0.05）. The CMCase and xylanase activities on complete yeast medium were significantly decreased than those on media without glucose （P〈0.05） or simple nitrogen sources （P〈0.05）. The laccase activities on media without simple nitrogen sources were significantly lower than those on complete yeast media （P〈0.05） and media without glucose （P〈0.05）, indicating the laccase activities on complicated media were lower than those on media with simple carbon and nitrogen sources.

关 键 词：羧甲基纤维素酶 木聚糖酶 漆酶 液体发酵 

分 类 号：S646.15[农业科学—蔬菜学]