响应面试验优化超声辅助提取柚皮纤维素工艺  

Optimization of Ultrasonic-Assisted Extraction of Cellulose from Pomelo Peel by Response Surface Methodology

作　　者：曾小峰[1] 白小鸣 盖智星 彭雪娇[1] 王华[1,3] 

机构地区：[1]西南大学柑桔研究所,重庆400712 [2]西南大学食品科学学院,重庆400715 [3]国家柑桔工程技术研究中心,重庆400712

出　　处：《食品科学》2015年第14期34-38,共5页Food Science

基　　金：重庆市应用开发计划项目(cstc2013yykf B00001);公益性行业(农业)科研专项(201503142)

摘　　要：以柚皮为原料研究超声辅助提取纤维素的工艺条件,通过单因素试验,分别考察氢氧化钠质量浓度、提取温度、提取时间及过氧化氢体积分数对柚皮纤维素含量的影响。在此基础上,应用响应面分析氢氧化钠质量浓度、提取温度、提取时间及三者两两交互作用对响应值的影响,确定了柚皮纤维素提取的最佳工艺参数。结果表明:各因素对纤维素含量影响的显著性表现为提取温度>氢氧化钠质量浓度>提取时间,通过响应面法优化的最佳工艺条件为氢氧化钠质量浓度为9.4 g/100 m L、提取温度为84℃、提取时间为77 min,在此条件下柚皮纤维素含量为63.12%。The present study optimized the ultrasonic-assisted extraction of cellulose from pomelo peel by the combined use of single factor method and response surface methodology （RSM）. In the extraction process, pomelo peel was ultrasonically treated with （NaOH） at a certain temperature for the removal of hemicelluloses, followed by further removal of lignin and simultaneous decolorization with hydrogen peroxide. The influences of sodium hydroxide concentration, extraction temperature, ultrasonication time and hydrogen peroxide concentration, as well as interactions among the former three variables on the cellulose content （i.e., purity） of pomelo peel extract were investigated. The results indicated that the cellulose content of pomelo peel extract was affected in decreasing order by the following factors： extraction temperature 〉 sodium hydroxide concentration 〉 ultrasonication time. Through response surface methodology, the process conditions for hemicelluloses removal were optimized as follows： NaOH concentration 9.4 g/100 mL, temperature 84 ℃ and ultrasonication time 77 rain. Under these conditions, the purity of cellulose extracted from pomelo peel was 63.12%.

关 键 词：柚皮 纤维素 超声波提取 响应面 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]