果汁原浆中葡萄糖含量测定的酶电极设计  

Development of Enzymatic Electrode for the Detection of Glucose in Not-From-Concentrate(NFC) Fruit Juices

作　　者：綦翠华[1] 李一苇[2,3] 张晓伟[1] 

机构地区：[1]济南大学酒店管理学院,山东济南250022 [2]山东省科学院生物研究所,山东省生物传感器重点实验室,山东济南250014 [3]济南大学化学化工学院,山东济南250022

出　　处：《食品科学》2015年第14期111-117,共7页Food Science

基　　金：山东省自然科学基金项目(ZR2014BP015);国家自然科学基金面上项目(21375047)

摘　　要：利用易于制备的水不溶性普鲁士蓝制备了介体掺入式丝网印刷电极,并以该电极作为电化学换能器制备葡萄糖氧化酶电极。电化学表征结果显示,酶电极对葡萄糖的电流响应特征良好,其线性范围为8.0?μmol/L^2.2?mmol/L,检测限达0.082?7?μmol/L(RSN=3),灵敏度为1.016?μA·L/mmol,响应时间5?s,其抗干扰性能、稳定性及重复性良好。实验还确定了该酶电极的最佳制备配方及工作条件。该电极制作简单、廉价,适用于成分复杂的果汁原浆的快速分析测试。A glucose oxidase （GOx） electrode fabricated on the basis of an electron mediator bulk-modified screen-printed electrode as an electrochemical transducer made from water-insoluble prussian blue （PB）, which was easy to prepare, was reported in this study. Electrochemical characterizations revealed that the enzyme electrode exhibited robust current response properties. It possessed a linearity toward glucose within the range of 8.0 μmol/L–2.2 mmol/L. Its limit of detection （LOD） was 0.082 7 μmol/L （RSN = 3）, sensitivity was 1.016 μA·L/mmol, response time was 5 s and it showed satisfactory antiinterference capability, stability and reproducibility. The formulation and working conditions of the electrode were also optimized. This electrode is easy to prepare, economic and applicable for the rapid assay of glucose in not-from-concentrate （NFC） fruit juices.

关 键 词：果汁原浆 葡萄糖 丝网印刷电极 工业应用 

分 类 号：TS3[轻工技术与工程]