采用反义寡核苷酸聚乳酸可降解微球建立小鼠肾阳虚模型  

Establishment of Mouse Models with Kidney-positive Deficiency with Antisense Oligonucleotide-loaded Biodegradable Polylactic Acid Microspheres

作　　者：李晓清[1] 应坚[1] 

机构地区：[1]重庆市中医院肿瘤科,重庆404100

出　　处：《中国药房》2015年第22期3086-3088,共3页China Pharmacy

摘　　要：目的:研究以反义寡核苷酸聚乳酸可降解微球建立小鼠肾阳虚模型的可行性。方法:取3周龄小鼠随机分为A、B、C、D、E组,每组6只,A组小鼠皮下注射0.2 ml生理盐水,其余组依次皮下注射反义寡核苷酸聚乳酸可降解微球0.1、0.2、0.4、0.8 mg/只。给药后,观察各组小鼠体质量和活动度,并于4周后采集其肝脏、肾上腺、脑等标本,采用实时定量聚合酶链反应(RT-PCR)法测定其中糖皮质激素受体(GR)表达情况。结果:除A组外,其余各组小鼠均见发育迟缓、体质量偏轻、活动减少、扎堆等肾阳虚证表现;与A组比较,其余组小鼠肝脏、肾上腺、脑中GR表达均降低,差异具有统计学意义(P<0.05)。结论:反义寡核苷酸聚乳酸可降解微球可抑制GR表达,GR表达被抑制的小鼠呈现肾阳虚证的临床表现。本方法可为小鼠肾阳虚模型的建立提供参考。OBJECTIVE：To investigate the feasibility of mouse models establishment with kidney-positive deficiency with antisense oligonucleotide-loaded biodegradable polylactic acid microspheres.METHODS：Three-week old mice were randomized into groups A,B,C,D and E,with 6 mice in each group,where the mice in group A were given subcutaneous injection 0.2 ml normal saline,and those in groups B-E were given subcutaneous injection 0.1,0.2,0.4 and 0.8 mg each mice of antisense oligonucleotide-loaded biodegradable polylactic acid microspheres.After the administration,the weights and activities of mice in all groups were observed;and one month later,their livers,adrenal glands and brains were collected to detect the expression of glucocorticoid receptor（GR） by real-time quantitative polymerase chain reaction（RT-PCR）.RESULTS：All mice except those in group A were found to have kidney-positive deficiency symptoms such as developmental delay,light weight,less activities and gathering together.Compared to group A,other groups showed lower GR expressions in liver,adrenal gland and brain.There was statistically difference（P〈0.05）.CONCLUSIONS：Antisense oligonucleotide-loaded biodegradable polylactic acid microspheres can inhibit GR expression,and the mice whose GR expression is inhibited show symptoms of kidney-positive deficiency,which provides a reference for the establishment of mouse models with kidney-positive deficiency.

关 键 词：反义寡核苷酸 聚乳酸可降解微球 小鼠 肾阳虚证 糖皮质激素受体 

分 类 号：Q95-331[生物学—动物学] R228[医药卫生—中医基础理论]