脂多糖、TNFα、IL-6、地塞米松和胰岛素对人乳腺癌MCF7细胞GPR54表达的影响  

Lipopolysaccharide,TNFα,IL-6,dexamethasone,and insulin increase the expression of GPR54 in the MCF7 breasr cancer cell line

作　　者：黄炳昆 茅江峰[1] 孙昭[2] 韩钦[3] 聂敏[1] 伍学焱[1] 

机构地区：[1]北京协和医院内分泌科/卫生部内分泌重点实验室,北京100730 [2]北京协和医院肿瘤内科,北京100730 [3]中国医学科学院基础医学研究所,北京100730

出　　处：《中华男科学杂志》2015年第7期587-592,共6页National Journal of Andrology

基　　金：国家自然科学基金青年基金(81100416)~~

摘　　要：目的:GPR54是下丘脑神经元调控下丘脑-垂体-性腺轴功能的门控,本文研究不同浓度脂多糖(LPS)、肿瘤坏死因子α(TNFα)、白细胞介素6(IL-6)、地塞米松和胰岛素对人乳腺癌MCF7细胞GPR54 mRNA及蛋白表达的影响。方法:培养MCF7细胞,用LPS(10μg/ml和20μg/ml)、TNFα(20 ng/ml和100 ng/ml)、IL-6(10 ng/ml和20 ng/ml)、地塞米松(10-6mol/L和10-7mol/L)和胰岛素(0.01 IU/L和0.1 IU/L)干预72 h,评价干预6、24、48、72 h后GPR54 mRNA(实时荧光定量PCR)和蛋白表达量(Western印迹)的变化。结果:和对照组相比,LPS(10μg/ml和20μg/ml)、TNFα(20 ng/ml和100 ng/ml)、IL-6(10 ng/ml和20 ng/ml)、地塞米松(10-6mol/L和10-7mol/L)和胰岛素(0.01 IU/L和0.1 IU/L)均显著增加GPR54 mRNA(P均<0.05)和蛋白表达。结论:LPS、TNFα、IL-6、地塞米松和胰岛素显著增加MCF7细胞GPR54的表达,提示这些炎症因子和激素可能通过调节GPR54水平变化进而影响性腺轴功能。Objective： To investigate the effects of different concentrations of lipopolysaccharide（ LPS）,tumor necrosis factorα（ TNFα）,interleukin-6（ IL-6）,dexamethasone（ Dex）,and insulin on the mRNA and protein expressions of GPR54 in the MCF7 cell line in vitro. Methods： MCF7 breasr cancer cells were cultured and treated with different concentrations of LPS（ 10 and20 μg / ml）,TNFα（ 20 and 100 ng / ml）,IL-6（ 10 and 20 ng / ml）,Dex（ 10^- 6and 10^- 7mol / L）,and insulin（ 0. 01 and0. 1 IU / L）. Those treated with culture fluid only served as controls. The mRNA and protein expressions of GPR54 were measured by real-time PCR and Western blot,respectively,after 6,24,48,and 72 hours of treatment. Results： Compared with the blank control,LPS（ 10 and 20 μg / ml）,TNFα（ 20 and 100 ng / ml）,IL-6（ 10 and 20 ng / ml）,Dex（ 10^- 6and 10^- 7mol / L）,and insulin（ 0. 01 and 0. 1 IU/L） significantly increased the expressions of GPR54 mRNA（ P〈0. 05） and protein（ P〈0. 05）. Conclusion：LPS,TNFα,IL-6,Dex,and insulin evidently increase the expression of GPR54 in the MCF7 cell line,indicating their influence on the function of gonads by regulating the GPR54 level.

关 键 词：GPR54 MCF7细胞系 脂多糖 地塞米松 胰岛素 白细胞介素6 肿瘤坏死因子Α 

分 类 号：R737.9[医药卫生—肿瘤]