PCDH 10基因启动子区高甲基化在结肠癌阶段性发生及进展中的作用  

The role of PCDH 10 gene promoter hypermethylation in staged occurrence and development of colon cancer

作　　者：刘美玲[1] 杨帆[2] 李冯锐[3] 包乌仁吐亚[3] 刘扬[3] 吴海荣[3] 杨占君[3] 

机构地区：[1]包头医学院第一附属医院,内蒙古包头014010 [2]包头市人民检察院 [3]包头医学院

出　　处：《包头医学院学报》2015年第7期1-2,11,共3页Journal of Baotou Medical College

基　　金：内蒙古自治区高等学校科学研究项目(NJZY13240)

摘　　要：目的:本研究通过检测结肠癌组织中PCDH 10基因启动子区Cp G岛甲基化状态,探讨PCDH 10基因启动子区异常甲基化在结肠癌的阶段性发生及进展中的作用。方法:采用甲基化特异性聚合酶链式反应(methylation-specific polymerase chain reaction,MSPCR)检测56例结肠癌及癌旁结肠组织、15例正常结肠组织中PCDH 10基因启动子区Cp G岛甲基化的状态,同时分析其与结肠癌临床特征之间的联系。结果:结肠癌、癌旁结肠组织及正常结肠组织中PCDH 10基因启动子区Cp G岛异常甲基化率分别为57.1%(32/56)、10.7%(6/56)和0%,三组之间比较,差异具有统计学意义(P<0.05)。PCDH 10基因启动子区Cp G岛甲基化状态与结肠癌的临床分期、结肠癌的分化程度以及淋巴结转移显著相关(P<0.05)。结论:PCDH 10基因启动子区异常甲基化是结肠癌组织区别于正常结肠组织的分子机制之一,可能在结肠癌的阶段性发生、发展中起着关键的作用。PCDH 10基因启动子区Cp G岛甲基化可能成为一种新的结肠癌肿瘤标志物。Objective： To explore the role of methylation abnormality of PCDH 10 gene promoter in the staged occurrence and development of colon cancer by detecting the methylation status of PCDH10 gene promoter Cp G island from the tissues of colon cancer. Methods： The methylation-specific polymerase chain reaction（ MSP） was applied to test methylation status of the PCDH 10 gene promoter Cp G island of the colon tissues and the adjacent colon tissues in 56 cases of colon cancer,and of the normal colon tissues in 15 cases,analyzing its association with clinical features of colon cancer. Results： The tissues and adjacent colon tissues of the colon cancer and normal colon tissues were examined and quantified.The rates of PCDH 10 gene promoter Cp G island methylation abnormality were 57. 1%（ 32 /56）,10. 7%（ 6 /56） and 0% among the three groups,respectively. The differences were statistically significant（ P〈0. 05）. The methylation status of PCDH 10 gene promoter Cp G island was significantly correlated to the clinical stages,the differentiated degree,and lymph node metastasis of colon cancer（ P〈0. 05）. Conclusion： PCDH 10 aberrant gene promoter methylation,indicated as one of the molecular mechanisms which differentiates normal colon tissues from colon cancer tissues,may play a key role in the staged occurrence and development of colon cancer. PCDH 10 gene promoter Cp G island methylation may have the potential as a new tumor diagnosis marker for colon cancer.

关 键 词：结肠癌 甲基化 PCDH 10基因 

分 类 号：R735.35[医药卫生—肿瘤]