Sulindac对人肝癌细胞HepG2增殖及β-catenin表达的影响  

The effect of Sulindac on proliferation and β-catenin protein expression of human liver cancer cell HepG2

作　　者：邓晓慧[1] 张应花[1] 方方[1] 宋海岩[1] 付升旗[1] 

机构地区：[1]新乡医学院解剖学教研室,河南新乡453003

出　　处：《中国临床解剖学杂志》2015年第4期447-450,共4页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金项目(81301174);新乡医学院培育基金项目(2014QN118)

摘　　要：目的观察Sulindac对人肝癌细胞HepG2增殖、凋亡及β-catenin蛋白表达的影响,探讨Sulindac抗肝癌的可能机制。方法不同浓度的Sulindac作用HepG2细胞后,采用MTT实验检测细胞增殖抑制作用;采用Hoechst33258染色法检测Sulindac对HepG2细胞凋亡的影响;利用RT-PCR及Western blotting检测HepG2细胞在Sulindac作用后Wint通路中β-catenin的表达变化。结果 Sulindac对人肝癌细胞HepG2有增殖抑制作用,且呈剂量时间依赖关系;Hoechst33258结果显示,Sulindac作用24 h后HepG2细胞凋亡数目明显增多;随着Sulindac浓度的增加,β-catenin mRNA及蛋白表达量逐渐下降。结论 Sulindac能够抑制人肝癌细胞HepG2增殖,通过阻断Wnt信号传导通路,降低β-catenin表达,诱导人肝癌细胞HepG2的凋亡。Objective To observe the effect of Sulindac on proliferation, apoptosis of human liver cancer cell HepG2 and the expression of β-catenin protein, and to investigate the possible anti-cancer mechanism of Sulindac. Methods HepG2 cells were treated with different dose of Sulindac. The inhibitory effect of Sulindac on proliferation was detected by MTT assay. Hoechst 33258 staining was applied to analyze the effect of Sulindac on apoptosis of HepG2 cell. The expression of β-catenin protein in the Wnt pathway was detected by RT-PCR and Western blotting. Results Sulindac could inhibit the proliferation of liver cancer cell HepG2 with time and dose dependence. The number of apoptotic cells after treated with Sulindac for 24h was larger than those treated with DMSO. The expression of β-catenin appeared to decrease with the increase of drug concentration. Conclusion Sulindac can inhibit the growth of liver cancer HepG2 cells. It can block the Wnt signal transduction pathway, and reduce the expression of β-catenin, which may be the mechanism of its anti-tumor effect.

关 键 词：舒林酸 肝癌 细胞凋亡 

分 类 号：R73-36[医药卫生—肿瘤]