响应面分析法优化假单胞菌产絮凝剂的培养条件  

Optimal culture conditions for Pseudomonas flocculant using response surface analysis

作　　者：何建玲[1] 杨双双[1,2] 黄金田[1] 周冰倩[1,3] 董锐[1] 

机构地区：[1]盐城工学院化学与生物工程学院,江苏盐城224051 [2]安徽理工大学化学工程学院,安徽淮南232000 [3]常州大学材料科学与工程,江苏常州213000

出　　处：《食品工业科技》2015年第16期183-187,192,共6页Science and Technology of Food Industry

基　　金：苏北科技发展计划--科技富民强县项目(BN2014049)

摘　　要：从油漆废水中分离得到一株具有较高絮凝活性的菌种FL-1,通过16s DNA凝胶电泳图鉴定菌种的种类为门多萨假单胞菌。然后采用单因素实验对培养基进行优化,分别考察了培养基的初始p H、碳氮源及温度对絮凝效果的影响。最后用响应面设计法对这四因素进行优化,得到最佳的培养条件为葡萄糖26.5 g/L,(NH4)2SO40.5 g/L,初始p H7.0,温度31℃。在此条件下的絮凝率的理论值为96.55%,实验验证值为95.19%。实验值与响应面预测值拟合情况良好,则说明通过响应面实验设计对FL-1培养条件的优化是有效的。Abstract.FL-1 bacterium was isolated from waste water,it had high flocculating activity. It was identified as Pseudomonas mendocina by 16sDNA gel electrophoresis figure. Then single factor experiments were adopted to optimize the cultural conditions,including pH,arbon nitrogen source and temperature. At last,response surface analysis was used to optimize above mentioned four factors. The optimum culture conditions were determined as follows：initial pH of 7.0,glucose concentration of 26.5 g/L, （NH4）2SO4 concentration of 0.5 g/L, cultivating temperature of 31 ℃. Under this culture conditions,the theoretical value of the flocculation rate was 96.55% ,and the test value was 95.19%. These results indicated that the experimental results were totally in accordance with the predictive results of response surface analysis which was a valid method to optimize the culture conditions of FL-1.

关 键 词：响应面法 假单胞菌 微生物絮凝剂 培养条件 

分 类 号：TS201.2[轻工技术与工程—食品科学]