石榴皮多酚调控肝细胞内胆固醇平衡的相关通路研究  

Effect of pomegranate peel polyphenols on regulation pathways of cholesterol in hepatic cell

作　　者：吕欧[1] 李建科[1] 马倩倩[1] 梁俊[1] 赵伟[1] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710062

出　　处：《食品工业科技》2015年第16期222-226,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(31171677)

摘　　要：探讨石榴皮多酚对成人肝细胞脂滴形成的影响,对肝细胞胆固醇合成限速酶基因及低密度脂蛋白受体基因表达的调控作用及其机制。以石榴皮多酚纯化物(PPPs)、安石榴苷(PC)和石榴鞣花酸(EA)为受试物,以体外培养成人正常肝细胞L-02为研究对象,采用油红O染色法观察肝细胞内脂滴变化情况,利用实时荧光定量核酸扩增(realtime quantitative,RT-PCR)分析对肝细胞胆固醇合成限速酶关键基因HMGCR m RNA、SE m RNA及肝细胞低密度脂蛋白受体基因LDLR m RNA及其上游基因SREBP-2 m RNA表达的影响。结果表明:石榴皮多酚三种受试物均能减少细胞内脂滴的形成,以石榴鞣花酸效果最好;与模型组相比较,石榴皮多酚各处理组均可极显著降低(p<0.01)肝细胞内胆固醇含量;与正常组相比较,石榴皮多酚三种受试物均可使胆固醇合成关键酶HMGCR和SE基因表达调控至较低水平,对LDLR基因和SREBP-2基因表达则有明显的上调作用。石榴皮多酚及其主要成分(安石榴苷和石榴鞣花酸)降低肝细胞胆固醇及降脂作用的机制可能是通过下调胆固醇合成限速酶基因表达,上调肝细胞低密度脂蛋白受体基因表达而实现的。This paper was aimed to study the effects of pomegranate peel polyphenols on lipid droplet formation of hepatocyte and the expression levels of cholesterol synthesis enzyme gene and low density lipoprotein receptor gene. Pomegranate peel polyphenols （PPPs）, punicalagin （PC） and pomegranate ellagic acid （EA） were used as test substances,L-02 human hepatocytes were choosen as experimental cell. The lipid droplets in human hepatocytes were assessed by oil red O staining,and the expression levels of HMGCR mRNA,SE mRNA,Iow density lipoprotein receptor gene LDLR mRNA and its upstream gene SREBP-2 mRNA was analyzed by RT-PCR. The results showed that these three test substances could reduce the intracellular lipid droplets,and the EA showed better effect. Compared with the model group,PPPs could significantly decrease （p〈0.01） cholesterol. Moreover,compared with the normal group,HMGCR mRNA and SE mRNA were down- regulated,whereas LDLR mRNA and SREBP-2 mRNA were up-regulated by pomegranate peel polyphenols. The possible mechanism of regulating cholesterol balance by PPPs and its mian component PC and EA in hepatic cells was via the downregulating cholesterol synthesis enzyme gene expression and upregulating the LDLR gene expression.

关 键 词：石榴皮多酚纯化物 安石榴苷 石榴鞣花酸 胆固醇 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]