重楼叶多糖改善D-半乳糖衰老模型小鼠脾脏免疫功能和抗氧化能力  

Effect of polysaccharides from the leaves of Paris polyphylla on immune function and antioxidant capacities of mouse spleen tissue in a D-galactose-induced aging mouse model

作　　者：刘功成[1] 王作伟[1] 李亭亭[1] 杜金莹[1] 杜小刚[1] 曹晓涵[1] 曾宪垠[1] 

机构地区：[1]四川农业大学生命科学院原子能农业应用研究室,四川雅安625014

出　　处：《食品工业科技》2015年第16期366-369,383,共5页Science and Technology of Food Industry

摘　　要：采用D-半乳糖注射诱导衰老模型,探讨了重楼叶多糖(PPLPs)对小鼠脾脏免疫功能和抗氧化的影响。分别对小鼠D-半乳糖、D-半乳糖和PPLPs、D-半乳糖和VC以及生理盐水处理,持续42 d。结果显示:与模型组相比,灌胃PPLPs或VC均能显著增加小鼠脾脏指数(p<0.05),显著上调脾脏免疫相关基因(T-bet、GATA、IFN-γ、IL-2、TNF-α、IL-4和IL-10)m RNA表达水平(p<0.05),也显著增强了脾脏总超氧化物歧化酶(T-SOD)、铜锌超氧化物歧化酶(Cu Zn-SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性(p<0.05);同时,灌胃PPLPs或VC显著降低了脾脏丙二醛(MDA)含量(p<0.05)。结果表明,PPLPs可增强脾脏免疫功能并提高小鼠的抗氧化活性。In the present study,the protective effects of polysaccharides from the leaves of Paris polyphylla（PPLPs） against aging in an accelerated aging mice model induced by D-galactose were investigated. A group of 2-month-old C57BL/6J mice were treated daily with D-galactose,D-galactose combined with PPLPs, D-galactose combined with vitamin C（Vc）,and control buffer for 42 d. The results showed that compared with the model group, oral administration of D-galactose-induced aging mice with either PPLPs or Vc both significantly increased spleen indices（p〈0.05）,the mRNA expression levels of immune function related genes （T-bet, GATA, IFN-γ, IL-2, TNF-α, IL-4, and IL- 10） （p〈0.05） and the total superoxide dismutase （T-SOD）, CuZn superoxide dismutase（CuZn-SOD）,glutathione peroxidase（GSH-Px） and catalase （CAT） in spleen tissue were significantly higher in both PPLPs and Vc group than those in the model group（p〈0.05）;besides, the malondialdehyde（MDA） concentrations were decreased in PPLPs and Vc group compared to those in the model group（p〈0.05）. The results indicated that PPLPs was able to enhance the immune function and improve the antioxidant capacities of mouse spleen tissue.

关 键 词：重楼多糖 衰老 免疫 抗氧化 脾脏 

分 类 号：TS201.1[轻工技术与工程—食品科学]