Tong xie yao fang relieves irritable bowel syndromein rats via mechanisms involving regulation of5-hydroxytryptamine and substance P  

出　　处：《World Journal of Gastroenterology》2015年第15期4555-4563,共9页世界胃肠病学杂志（英文版）

基　　金：Supported by National Education Department“ChunHui Plan”Research Projects,No.Z2010021;China Postdoctoral Science Foundation Project,No.2013M531079;Heilongjiang Postdoctoral Funding Project,No.LBH-Z12246;Heilongjiang Education Department Scientific Research Project,No.12521502;excellent Innovative Talents Support Program Funding of Heilongjiang University of Chinese Medicine(Outstanding Young Academic Leaders),No.051217

摘　　要：AIM To study the effect of hydrogen sulfide （H2S） onsevere acute pancreatitis （SAP） in a rat model.METHODS： Sprague-Dawley （SD） rats were administeredan intraperitoneal injection of saline containing20% L-Arg （250 mg/100 g） hourly for over 2 h to induceSAP. The rats were treated with DL-propargylglycine（PAG, 50 mg/kg） or different dosages of NaHS （5 mg/kg, 10 mg/kg, 20 mg/kg or 100 mg/kg）. PAG or NaHSwas administered 1 h before induction of pancreatitis.Rats were sacrificed 24 h after the last L-Arg injection.Blood and pancreas tissues were collected.RESULTS： The H2S and cystathionine-γ-lyase mRNAlevels in SAP rats were signi？cantly lower than thosein the control group, and treatment with PAG furtherreduced the H2S level. Nevertheless, H2S was significantlyincreased after NaHS administration compared with theSAP group, and the degree of upregulation was associatedwith the NaHS dosage. NaHS reduced the levels of plasmaamylase, interleukin-6 and myeloperoxidase in pancreatictissue. NaHS suppressed the degradation of IκBα and theactivity of nuclear factor-κB, as well as the phosphorylationof PI3K/AKT.CONCLUSION： H2S plays an anti-inflammatory role inSAP in vivo .

关 键 词：severe acute PANCREATITIS Hydrogen SULFIDE CYSTATHIONINE-Γ-LYASE CYTOKINE signaling pathway 

分 类 号：R[医药卫生]