纤维素酶产生菌及其发酵条件优化  

Screening of cellulase-producing strain and optimization of fermentation conditions

作　　者：刘丹[1] 王红英[1] 吴星[1] 钱斯日古楞 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《工业微生物》2015年第4期19-25,共7页Industrial Microbiology

基　　金：内蒙古自治区科技重大专项资助

摘　　要：通过刚果红染色产脱色圈初筛出能分解纤维素的菌株,再利用DNS法分别测定纤维素酶的酶活,得到分解纤维素能力最强的一株真菌Lv-1。该菌株菌丝为黄绿色,孢子为深绿色,有多分支的无隔菌丝,长孢子囊孢子,用DNS法测定其酶活为62.42 U/m L,后对其进行了产酶条件优化。经单因素试验和正交试验得到该菌的最佳产酶条件为:在以10 g/L羧甲基纤维素钠为碳源,4 g/L蛋白质,2 g/L硫酸铵为氮源,1 g/L硫酸二氢钾为无机盐的最适产酶培养基中,初始p H为6.0,培养温度37℃,装液量100 m L/250 m L,发酵36 h。在此发酵条件下,其产酶活力可达96.898U/m L,试验结果显示,该菌株在产纤维素酶能力上具有显著优势,且菌株Lv-1产酶活力较优化前高出34.478 U/m L,提高了55.24%。Several strains with the ability of decomposing cellulose were screened out by congo red identification agar plate, and then their cellulase activities were determined by DNS method to get a strain with high extracellular cellulase productivity, a kind of fungi named Lv-1 with 62.42 U/mL of enzyme activity. Based on the single factor experiments and the orthogonal experiments, the optimum fermentation conditions for cellulase Lv-1 production were obtained as follows ： 10 g/L CMC-Na, 4 g/L protein, 2 g/L （NH＋）2SO4, 1 g/L KH2SO4, initial pH 6.0, culture temperature 37 ℃, loading volume 50 mL/250 mL and fermentation time 36 h. Under the optimized conditions, the cellulase activity of strain Lv-1 reached 96. 898 U/mL, increased by 34. 478 U/mL, up to 55.24% than before.

关 键 词：纤维素酶 筛选 正交试验 酶活 

分 类 号：TQ925[轻工技术与工程—发酵工程]