响应面优化生物素亚适量法生产谷氨酸工艺  

The Response Surface to Optimize Production of Glutamic Acid by Appropriate Biotin Method

作　　者：张金玲[1,2] 朱思荣[1,2] 周万里[1,2] 史建国[1,2] 赵晓华[1,2] 毕春元[1,2] 

机构地区：[1]山东省科学院生物研究所,济南250014 [2]山东省生物传感器重点实验室,济南250014

出　　处：《中国调味品》2015年第8期15-18,23,共5页China Condiment

基　　金：山东省自然基金项目(ZR2011CL008)

摘　　要：基于生物量在线检测技术对谷氨酸发酵工艺进行研究,经Plackett-Burman试验设计发现硫酸镁、维生素B1和玉米浆为三个影响谷氨酸产酸的主要因素。采用最陡爬坡实验逼近最大响应区,借助Box-Behnken试验设计进行响应面优化回归分析,探讨该工艺的最佳条件值,结果显示:谷氨酸产酸最大理论值为86.52g/L,硫酸镁最佳浓度4.26g/L,维生素B1最佳浓度18.03mg/L,玉米浆最佳浓度33.91mL/L,在10L发酵罐S9114谷氨酸棒状杆菌32℃发酵实验上验证,实测值与理论值呈现很好的一致性,在优化条件下谷氨酸产酸提高了19.7%,为谷氨酸发酵提供了参考。Based on biomass on-line detection technology of glutamic acid fermentation, a process is studied through Plackett-Burman test design, it is found that magnesium sulfate, vitamin B1 and corn starch as the three main factors affect glutamic acid production. Approaching the maximum response of the steepest uphill experiment area, a Box-Behnken experimental design is applied to analyze the response surface optimization, investigate the best conditions of the process value and the results show that the maximum theoretical value of glutamic acid yield is 86.52 g/L, the optimal concentration of magnesium sulfate, vitamin B1 and corn slurry is 4.26 g/L, 18.03 rag/L, 33.91 mL/L respectively, which is verified by the fermentation experiment in 10 L fermentation tank with S9114 glutamate corynebacterium at 32 ℃, the measured values and the theoretical values are close to. Under the condition, the glutamic acid yield is increased by 19. 7%, provides reference for glutamic acid fermentation.

关 键 词：谷氨酸发酵 响应面优化 生物素亚适量 PLACKETT-BURMAN Box-Behnken 

分 类 号：TS264.23[轻工技术与工程—发酵工程]