在线检测在谷氨酸发酵中的应用  

The Application of Online Detection in Glutamic Acid Fermentation

作　　者：杜祎[1] 朱思荣[2,3] 周万里[2,3] 史建国[2,3] 赵晓华[2,3] 毕春元[2,3] 

机构地区：[1]齐鲁工业大学生物工程学院,济南250353 [2]山东省科学院生物研究所,济南250014 [3]山东省生物传感器重点实验室,济南250014

出　　处：《中国调味品》2015年第8期116-118,共3页China Condiment

基　　金：国家"863"计划项目(2012AA021201);山东省自然基金项目(ZR2011CL008)

摘　　要：谷氨酸发酵过程是一个复杂的、不确定的、非线性的动态过程,其影响因素很多,机理十分复杂。以高端C8051F120(SOC)单片机为核心部件,采用计算机控制实现生物量在线检测可减轻操作人员的劳动强度,提高发酵系数和经济效益。选择1‰葡萄糖-谷氨酸溶液作为标准液,采用嵌入式、可编程逻辑的微控制器,基于集散控制、工业控制的计算机发酵测控系统对10L发酵罐谷氨酸棒状杆菌S9114在32℃条件下32h的发酵实验进行检测。结果与离线测定比较呈现很好的一致性。该系统及时、准确地反映了发酵过程葡萄糖、谷氨酸等生物量浓度随时间的变化。Glutamic acid fermentation process is a complex, uncertain and nonlinear dynamic process, many factors that affect it and its mechanism is very complex. Take high-end C8051F120 （SOC） single-chip microcomputer as the core component, using computer control to realize biomass on-line detection can reduce the labor intensity of operators, improve the coefficient of fermentation and economic benefits. The fermentation control system options 1‰ glucose-glutamic acid solution as the standard and uses embedding, micro programmable logic controller, and bases on distributed control, industrial control computer measurement to test the glutamate corynebacterium S9114 fermentation experiment at 32 ℃ in 10 L fermentation tank for 32 hours. The results compared with off-line measurement show good consistency. The system timely and accurately reflects the biomass concentration of glutamic acid fermentation process, such as the change of glucose and glutamic acid with time.

关 键 词：谷氨酸 发酵 生物量 在线检测 

分 类 号：TS201.24[轻工技术与工程—食品科学]