首发精神分裂症患者调节性T细胞及相关细胞因子变化  

Circulating levels of Treg cells and cytokines in patients with first-episode schizophrenia

作　　者：黄满丽[1] 陈京凯 叶于富[1] 许毅[1] 胡少华[1] 胡健波[1] 周韦华[1] 魏宁[1] 戚洪莉[1] 许委娟[1] 

机构地区：[1]浙江大学医学院附属第一医院精神卫生科,浙江省精神障碍诊疗和防治技术重点实验室,杭州310003

出　　处：《浙江医学》2015年第14期1189-1192,共4页Zhejiang Medical Journal

基　　金：浙江省教育科技厅高效科研计划项目(Y201120182)

摘　　要：目的探讨首发精神分裂症患者调节性T细胞及细胞因子变化及与精神症状的关系。方法采用流式细胞仪技术检测27例首发精神分裂症患者和27例健康体检者外周血CD4^+CD25^+Foxp3^+调节性T细胞(Treg)占CD4^+T细胞的百分率,酶联免疫吸附试验检测血清IL-10及TGF-β1水平,采用阳性与阴性症状量表(PANSS)评定精神分裂症患者精神症状。Treg细胞、TGF-β1与年龄、病程、教育年限和精神症状以及细胞因子的相关性采用Spearman相关分析。结果首发精神分裂症患者Treg细胞百分率为(0.03±0.01)%,明显低于对照组(0.4±0.02)%(t=-3.681,P=0.001);首发精神分裂症患者血清TGF-β1[(1.40±1.16)pg/ml]水平明显高于对照组[(0.75±0.81)pg/ml](t=2.376,P=0.021),但IL-10水平在精神分裂症组[(0.12±0.25)pg/ml]和对照组[(0.12±0.26)pg/ml]间比较差异无统计学意义(t=0.432,P=0.668)。首发精神分裂症患者的Treg百分率与教育年限呈正相关(r=0.452,P=0.018),与年龄、病程和精神症状无相关性;TGF-β1与年龄、病程、教育年限和精神症状之间均无相关性。首发精神分裂症患者的Treg细胞百分率与IL-10、TGF-β1间均无相关性。结论首发精神分裂症存在免疫抑制机制受损,Treg及TGF-β1可能在首发精神分裂症患者免疫失衡的病理生理机制发挥着重要的作用。Objective To investigate the changes in the circulating levels of Treg cells and cytokines in patients with first-episode schizophrenia. Methods The fraction of circulating CD4±CD25±FOXP3± Treg cells, serum levels of interleukin-10 （IL-10） and transforming growth factor-13 1 （TGF-β1） in 27 first episode schizophrenia patients and 27 healthy controls were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. The positive and negative symptom scale （PANSS） scores were used to evaluate the psychotic symptoms. Results The first-episode schizophrenia patients showed a significantly lower level of circulating Treg cells （0.03 ± 0.01） than that in control subjects （0.04 ± 0.02）（t=-3.681, P=0.001）. Serum TGF-β 1 level was significantly higher （t=2.376, P=0.021） in first-episode schizophrenia patients（1.40 ± 1.16 pg/ml） than that in controls （0.75 ± 0.81pg/ml）. No statistical significance was detected in serum IL-10 level between two groups （0.12 ± 0.25 pg/ml, 0.12 ± 0.26 pg/ml, t=0.432, P=0.668）. Spearman analysis showed that the fraction of Treg cells was positive correlated with edu- cation （r=0.452, P=0.018）, not correlated to score of PANSS, age and course of disease. TGF- β1 was not correlated to score of PANSS, education, age and course of disease. The fraction of Treg cells was not correlated to TGF-β1 and IL-10. Conclusion A lower fraction of circulating Treg cells and higher level of TGF-β1 might be involved in the pathogenesis of schizophrenia.

关 键 词：精神分裂症 细胞因子 调节性T细胞 

分 类 号：R749.3[医药卫生—神经病学与精神病学]