参芎化瘀胶囊对全脑缺血大鼠海马区Notch1/GAP-43表达的影响  

Influence on the expression of Notch1/GAP-43 after global cerebral ischemia-reperfusion in hippocampus by Shenxiong Huayu Capsules

作　　者：周娜 赵雅宁 李建民 

机构地区：[1]华北理工大学护理与康复学院,河北唐山063000 [2]华北理工大学附属医院神经外科,河北唐山063000

出　　处：《中国医药导报》2015年第23期8-11,16,F0004,共6页China Medical Herald

基　　金：河北省自然科学基金资助项目(H2012401007)

摘　　要：目的探讨参芎化瘀胶囊对全脑缺血再灌注大鼠海马区Notch1/GAP-43表达的影响。方法将100只健康雄性SD大鼠随机分为假手术组（n=25）、脑缺血再灌注组（n=25）、参芎化瘀胶囊低剂量组（n=25）以及高剂量组（n=25）。参芎化瘀胶囊低剂量组以及高剂量组灌胃剂量为240、480 mg/（kg·d）,假手术组和脑缺血再灌注组按10 mL/kg体重给予蒸馏水,早上8∶00给药,每日1次,连续7 d。运用改良的Pulsineli 4血管阻断（4-VO）法进行全脑缺血再灌注模型的制作,分别于1、2、3、7、14 d断头处死取海马组织,HE染色观察海马细胞形态变化,免疫组化法检测Notch1、GAP-43的表达。造模后1-3 d采用横木行走实验评价大鼠感觉运动功能。结果与假手术组比较,脑缺血再灌注组海马区正常神经元计数明显减少,差异有统计学意义（P〈0.05）,Notch1（术后1、2、3 d）、GAP-43（术后3、7、14 d）表达均增多,差异有统计学意义（P〈0.05）,横木行走实验评分明显减少（P〈0.05）;与脑缺血再灌注组比较,参芎化瘀胶囊低剂量组正常神经元计数增多,Notch1、GAP-43蛋白明显增多（P〈0.05）,横木行走实验评分明显提高（P〈0.05）。参芎化瘀胶囊高剂量组上述各指标变化表现得更加明显（P〈0.05）。脑缺血再灌注组和参芎化瘀胶囊低剂量组中Notch1和GAP-43表达均呈正相关（r=0.838,P〈0.01;r=0.786,P〈0.01）。结论参芎化瘀胶囊可能通过Notch1/GAP-43通路对全脑缺血大鼠起神经保护作用。Objective To investigate the effect of Shenxiong Huayu Capsules on the expression of Notch1/GAP-43 after global cerebral ischemia reperfusion in hippocampus. Methods One hundred healthy male SD rats were randomly divided into 4 groups： sham operation group（n = 25）, cerebral ischemia reperfusion group（n = 25） and Shenxiong Huayu Capsules low-dose group（n = 25） and high-dose group（n = 25）. The gavage dose of Shenxiong Huayu Capsules lowdose group and high-dose group was 240, 480 mg/（kg·d）, the sham operation group and cerebral ischemia reperfusion group were given distilled water according to 10 mL/kg weight, once every 8 ∶00 am, for 7 days. The model of global cerebral ischemia reperfusion was made by improved four-vessel occlusion according to Pulsinelli 4 vascular occlusion（4-VO） method, and the rats were sacrificed at 1, 2, 3, 7 d and 14 d respectively after injury to take the hippocampus tissues. HE staining was used to detect morphological changes in hippocampus each point in time; and the expressions of Notch1（1, 2, 3 d） and GAP-43（3, 7, 14 d） were measured by immunohistochemistry. The sensorimotor functions of the rats were detected by beam walking test on 1-3 days after injury. Results Compared with sham operation group, the neuron counts of rats in cerebral ischemia reperfusion group was significantly decreased（P〈0.05）. The expressions of Notch1（1-3 days after injury） and GAP-43（3, 7, 14 d after injury） in cerebral ischemia reperfusion group were enhanced obviously at the corresponding time（P〈0.05）, and the scores of beam walking test were decreased obviously in cerebral ischemia reperfusion group（P〈0.05）. Compared with cerebral ischemia reperfusion group, the neuron counts were decreased obviously in Shenxiong Huayu Capsule low-dose group（P〈0.05）, the expressions of Notch1 and GAP-43 were furthe r increased in Shenxiong Huayu Capsules low-dose group（P〈0.05）, and the scores of beam walking test improved significantly in Shenxiong H

关 键 词：全脑缺血 参芎化瘀胶囊 NOTCH1 GAP-43 

分 类 号：R743.3[医药卫生—神经病学与精神病学]