冬虫夏草多糖抗氧化活性及其单糖组成的研究  

Antioxidant activities and monosaccharide composition analysis of Cordyceps sinensis polysaccharides

作　　者：张安强[1] 王小臣[1] 王星丽 瞿亮 孙培龙[1] 马新[1] 

机构地区：[1]浙江工业大学海洋学院,杭州310014 [2]杭州百山祖生物科技有限公司,杭州310052

出　　处：《食药用菌》2015年第4期237-242,共6页Edible and Medicinal Mushrooms

基　　金：十二五国家科技支撑项目课题"食用菌深加工产品开发与产业化"(2013BAD16B07)

摘　　要：利用超声辅助提取法和分级醇沉得到3种醇沉组分粗多糖CSP40、CSP60和CSP80,并分析这3种组分的抗氧化活性。活性较好的粗多糖CSP40经过DEAE-Sepharose Fast Flow离子柱层析和Sephacryl S-400凝胶柱层析纯化得到单一组分CSP40-2a,进行乙酰化处理后用GC-MS测得其单糖组成。结果表明,超声辅助提取所得的3个醇沉组分均具有较好的抗氧化活性,从强到弱依次为CSP40>CSP80>CSP60。乙酰化分析表明,CSP40-2a主要由3种单糖组成,分别是D-甘露糖、D-葡萄糖和D-半乳糖,其摩尔比为1.98︰1.0︰0.67。Three crude polysaccharide sections named CSP40, CSP60 and CSP80 were obtained by ultrasonic-assisted extraction and grading alcohol precipitation. Antioxidant activities of these three sections were tested and the most active section CSP40 was purified by DEAE-Sepharose Fast Flow and Sephacryl S-400 chromatography. The monosaccharide composition of the pure polysaccharide named CSP40-2a was analyzed by GC-MS after acetylation. Result showed that CSP40, CSP60 and CSP80 had a good ablility of antioxidant and the order was CSP40〉CSPS0〉CSP60. Acetylating analysis by GC-MS indicated that CSP40-2a was composed of D-Mannose, D-Glucose and D-Galactose at the rate of 1.98： 1.0： 0.67.

关 键 词：冬虫夏草 多糖 抗氧化活性 多糖纯化 单糖组成 

分 类 号：S567.35[农业科学—中草药栽培]