浓香型大曲中1株高液化力功能细菌的筛选与鉴定  

Isolation and Identification of a Bacteria from Luzhou-flavor Daqu

作　　者：陈晓旭[1] 明红梅[1] 罗惠波[1] 许德富[2] 朱莉莉[1] 姚霞[1] 薛登文 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]泸州老窖股份有限公司,四川泸州646000

出　　处：《贵州农业科学》2015年第7期110-113,共4页Guizhou Agricultural Sciences

基　　金：酿酒生物技术及应用四川省重点实验室项目"浓香型大曲优势菌的筛选及微生态制剂的研发"(NJ2011-13);泸州老窖科研奖学金项目"浓香型大曲中产香微生物的筛选及应用研究"(13ljzk04);四川省教育厅重点科研项目"浓香型大曲微生态特征及立体制曲工艺优化的研究"(15ZA0219);四川省大学生创新训练计划项目"浓香型大曲中产香菌的筛选及香曲制备研究"(201410622004)

摘　　要：为探寻大曲微生物与风味物质之间的相关性,为构建大曲功能菌群及实现制曲生产异地化奠定基础,采用平板筛选、固态发酵、16SrDNA鉴定及HS-SPME-GC-MS等方法对浓香型大曲中的高液化力菌株进行筛选与鉴定。结果表明:菌株X20与枯草芽孢杆菌的16SrDNA序列同源性高达99%,可确定其为枯草芽孢杆菌;该菌株的液化力高达11.756g/(g·h),其代谢产物含有吡嗪类物质、愈创木酚和苯甲醛等多种重要的大曲风味物质,可初步确定其为大曲的功能菌株。In order to filter out high stress liquefaction bacteria and provide references for Daqu functional bacteria flora construction, the authors used plate screening test, solid state fermentation experiment, 16S rDNA and HS-SPME-GC-MS to seek the relationship between microorganism in Daqu and flower compounds. Results： The bacterial strain X20 had the highest liquefaction ability, up to 11. 756 g/（g. h）,the 16S rDNA sequence about X20 and Bacillus subtilis homology as high as 99%, which could be identified as B. subtilis, the metabolites contained pyrazines, guaiacol, benzaldehyde and some other kinds of daqu important flavor. It could be initially identified as functional strain of Daqu.

关 键 词：浓香型大曲 高液化力 细菌 功能菌株 HS-SPME-GC-MS 16SrDNA 

分 类 号：S182[农业科学—农业基础科学] Q93-3[生物学—微生物学]