Gastrointestinal endoscopy biopsy derived proteomic patterns predict indeterminate colitis into ulcerative colitis and Crohn's colitis  

作　　者：Billy Ray Ballard Amosy Ephreim M'Koma 

机构地区：[1]Department of Pathology, Meharry Medical College School of Medicine [2]Department of Biochemistry and Cancer Biology, Meharry Medical College School of Medicine [3]Department of Surgery, Vanderbilt University School of Medicine [4]Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine

出　　处：《World Journal of Gastrointestinal Endoscopy》2015年第7期670-674,共5页世界胃肠内镜杂志（英文版）（电子版）

基　　金：Supported by NIH/NIDDK R21DK095186-01A1,Nos.3U54 CA091408–09S1,U54RR026140/U54MD007593,and UL1 RR024975;Research Foundation,American Society of Colon and Rectal Surgeons,Limited Project Grant(LPG-086)

摘　　要：Patients with indeterminate colitis(IC) are significantly younger at diagnosis with onset of symptoms before the age of 18 years with significant morbidity in the interim. The successful care of IC is based on microscopic visual predict precision of eventual ulcerative colitis(UC) or Crohn's colitis(CC) which is not offered in 15%-30% of inflammatory bowel disease(IBD) patients even after a combined state-of-the-art classification system of clinical, visual endoscopic, radiologic and histologic examination. These figures have not changed over the past 3 decades despite the introduction of newer diagnostic modalities. The patient outcomes after restorative proctocolectomy and ileal pouch-anal anastomosis may be painstaking if IC turns into CC. Our approach is aiming at developing a single sensitive and absolute accurate diagnostic test tool during the first clinic visit through endoscopic biopsy derived proteomic patterns. Matrix-assisted-laser desorption/ionization mass spectrometry(MS) and/or imaging MS technologies permit a histology-directed cellular test of endoscopy biopsy which identifies phenotype specific proteins, as biomarker that would assist clinicians more accurately delineate IC as beingeither a UC or CC or a non-IBD condition. These novel studies are underway on larger cohorts and are highly innovative with significances in differentiating a UC from CC in patients with IC and could lend mechanistic insights into IBD pathogenesis.Patients with indeterminate colitis （IC） are significantlyyounger at diagnosis with onset of symptoms before theage of 18 years with significant morbidity in the interim.The successful care of IC is based on microscopic visualpredict precision of eventual ulcerative colitis （UC） orCrohn’s colitis （CC） which is not offered in 15%-30% ofinflammatory bowel disease （IBD） patients even after acombined state-of-the-art classification system of clinical,visual endoscopic, radiologic and histologic examination.These figures have not changed over the past 3 decadesdespite the introduction of newer diagnostic modalities.The patient outcomes after restorative proctocolectomyand ileal pouch-anal anastomosis may be painstaking ifIC turns into CC. Our approach is aiming at developinga single sensitive and absolute accurate diagnostictest tool during the first clinic visit through endoscopicbiopsy derived proteomic patterns. Matrix-assisted-laserdesorption/ionization mass spectrometry （MS） and/orimaging MS technologies permit a histology-directedcellular test of endoscopy biopsy which identifiesphenotype specific proteins, as biomarker that wouldassist clinicians more accurately delineate IC as beingeither a UC or CC or a non-IBD condition. These novelstudies are underway on larger cohorts and are highlyinnovative with significances in differentiating a UCfrom CC in patients with IC and could lend mechanisticinsights into IBD pathogenesis.

关 键 词：INDETERMINATE ULCERATIVE Crohn’s colitis The colitides Proteomics Diagnostic accuracy 

分 类 号：R574.62[医药卫生—消化系统]