Genomic-SSR和EST-SSR在柱花草种间的遗传差异  

Comparative analysis of genetic diversity revealed by genomic-SSR and EST-SSR markers in interspecies of Stylosanthes

作　　者：丁西朋 罗小燕[1] 邵辰光[2] 张龙[1] 王文强[1] 白昌军[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《广东农业科学》2015年第14期106-113,共8页Guangdong Agricultural Sciences

基　　金：海南省自然科学基金(313067);中央级公益性科研院所基本科研业务费专项资金(1630032013002);国家现代产业体系牧草岗位科学家经费项目

摘　　要：为了探讨genomic-SSR与EST-SSR标记在柱花草种间的遗传差异,利用两种SSR标记技术,对来自不同种的12份柱花草种质进行了遗传分析,并对两种不同标记进行比较。结果表明:EST-SSR标记相比genomic-SSR在柱花草不同种间具有更好的通用性,EST-SSR检测到的等位基因数和PIC指数平均值分别是4.6和0.610,genomic-SSR检测到的等位基因数和PIC指数平均值分别是5.6和0.702。聚类结果表明,genomic-SSR和EST-SSR均能有效鉴别所有供试柱花草种质。To explore the genetic differences between genomic-SSR and EST-SSR in different species of Stylosanthes, the genetic diversity of 12 accessions from different Stylosanthes species was analyzed by two types of SSR markers. Results showed that, compared with genomic-SSR, EST-SSR markers were superior in versatility for different species of Stylosanthes. The average of number of alleles and the polymorphic information content （PIC） value detected by EST-SSR was 4.6 and 0.610, respectively. The average of number of alleles and PIC value detected by genomic-SSR was 5.6 and 0.702, respectively. Cluster analysis indicated that two SSR markers could effectively identify all Stylosanthes accessions.

关 键 词：柱花草 EST-SSR GENOMIC-SSR 遗传差异 

分 类 号：Q346.5[生物学—遗传学]