唐古特青兰总黄酮的提取及其体外抗氧化活性的研究  

Study on extraction of total flavonoids from Dracocephalum tanguticum and its antioxidant activity in vitro

作　　者：谢建锋[1] 朱林燕[1] 孔子铭 朱刚[1] 韩泳平[1] 

机构地区：[1]西南民族大学少数民族药物研究所,四川成都610041

出　　处：《华西药学杂志》2015年第4期422-424,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家十二五科技支撑计划(2012BAI27B07);西南民族大学研究生创新型科研项目(CX2015SZ039)

摘　　要：目的优化唐古特青兰总黄酮提取的方法,并考察其体外抗氧化活性。方法采用单因素法与正交试验法确定了超声波辅助提取的优化条件,并探讨了总黄酮的还原能力以及对羟基自由基和超氧阴离子的清除能力。结果最佳提取工艺条件为:提取温度70℃、乙醇浓度50%、料液比1∶35;总黄酮提取物的还原能力略低于抗坏血酸,而对羟基自由基和超氧阴离子的清除率高于后者。结论超声波辅助提取唐古特青兰总黄酮具有提取率高,操作简便的特点,提取物的抗氧化活性较为明显,具有一定的实际应用价值。OBJECTIVE To study the optimum extraction conditions of total flavonoids from the Dracocephalum tanguticum Maxim and its antioxidant activity in vitro. METHODS The extraction conditions of ultrasonic method were determined by single factor and orthogonal test method. The reducing power and scavenging capacity of hydroxyl free radical and superoxide anion were investigated. RESULTS The optimum extraction conditions were as follows ： Temperature of 70 ~C , ethanol concentration of 50% and solid liquid ratio was 1 ： 35. The test showed that scavenging rate of total flavonoids extracts on hydroxyl radicals and peroxide anion radical were better than ascorbic acid, but the reducing power was less than latter. CONCLUSION This method is easy to operate with the high yield. The antioxidant activity of total flavonoids is obvious and worth promotion.

关 键 词：唐古特青兰 总黄酮 超声提取法 单因素法 正交试验 抗氧化活性 工艺优化 自由基清除 

分 类 号：R96[医药卫生—药理学]