石油烃降解菌CQ6产生物表面活性剂发酵条件的优化  

Optimization of Conditions for Petroleum-degrading Bacteria CQ6 Producing Biosurfactant

作　　者：付瑞敏[1,2] 杨雪[1] 谷亚楠[2] 薛婷婷[2] 邢文会[1] 张红[1] 张丽琴[1] 常慧萍[1] 陈五岭[2] 

机构地区：[1]河南教育学院生命科学系,郑州450046 [2]西北大学生命科学学院,西安710069

出　　处：《湖北农业科学》2015年第11期2710-2714,共5页Hubei Agricultural Sciences

基　　金：河南省科技攻关重点项目(122102310171);河南教育学院青年科研课题项目(20100103)

摘　　要：为了了解长庆油田石油烃降解菌产生物表面活性剂的情况,对菌株CQ6以原油为碳源时石油的降解率和发酵液表面张力进行了研究,并采用响应面分析法对该菌产生物表面活性剂的发酵条件进行了优化,在单因素的基础上,选择温度、转速和初始加油量3个因素,利用Box-Behnken中心组合原理和响应面分析法对数据进行了回归分析,得到了石油烃降解菌CQ6产生物表面活性剂的二次多项式回归方程的预测模型。结果表明,菌株CQ6产生物表面活性剂的最佳条件为温度25℃、转速190 r/min、接种量3.4%。在此条件下,菌株CQ6对石油的降解率可由优化前的64.4%升高至80.2%,菌株发酵液的表面张力由优化前的32.5 m N/m降至27.0 m N/m。In order to determine the surfactant-producing situation of petroleum-degradation bacteria in Changqing oil field, degradation rate of petroleum and surface tension of fermentation broth were studied when stain CQ-6 used crude oil as the carbon source, and the fermentation condition was optimized. Based on the single factor experiment, the temperature, the ro- tate speed and initial amount of oil as factors, the prediction model of quadratic regression polynomial equation on surfactant- producing was simulated with Box-Benhnken central composite principle and response surface methodology. The resuh showed that,the optimum condition of biosurfactant producing by strain CQ6 was as follows： the temperature was 25 °C ,the rotate speed was 190 r/min and the initial amount of oil was 3.4%.Under this condition, the degradation rate of petroleum increased from 64.4% to 80.2% and the surface tension of fermentation broth decreased from 32,5 mN/m to 27.0 mN/m compared with before optimization.

关 键 词：石油烃降解菌 生物表面活性剂 发酵 响应面分析 优化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]