诱导烟草悬浮细胞氧爆发的海绵共附生真菌HMP-F66的筛选与鉴定  

Screening and Identification of a Sponge-associated Fungus HMP-F66 Inducing Oxidative Burst in Tobacco Cell Suspensions

作　　者：庞志伟[1,2] 路旭[2,3] 胡江春[2] 陈晓琦[2,3] 王楠[2] 宋艳玲[1] 

机构地区：[1]沈阳化工大学制药与生物工程学院,沈阳110142 [2]中国科学院沈阳应用生态研究所,沈阳110016 [3]中国科学院大学,北京100049

出　　处：《生物技术通报》2015年第7期174-179,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31471814)

摘　　要：激发植物本身的天然免疫系统可作为防控植物病害的新途径。以50株分离自繁茂膜海绵的真菌为受试菌株筛选具有诱导植物抗性活性的菌株及其代谢产物。采用基于H2DCF-DA的荧光酶标法考察这些真菌的粗代谢物诱导烟草悬浮细胞产生氧爆发的活性,最终筛选到一株真菌HMP-F66能够显著诱导烟草悬浮细胞产生活性氧。根据其形态学特征、培养特征、特征性生理生化反应以及基于r DNA ITS的分子生物学分析,推测该菌株为米曲霉(Aspergillus oryzae)。The plant innate immune system provides an alternative way for crop protection, and the plant immunity triggers have been an important source of biorational pesticide leads. In order to find hey,. plant resistance inducers, fifty sponge-associated fungal isolates were screened for their ability to induce oxidative burst in the tobacco suspensions using the H2DCF-DA fluorescence assay. Strain HMP-F66 showed significant activity in inducing H2O2 production in tobacco suspensions. HMP-F66 was assumed to be Aspergillus oryzae by its morphological, physiological, cultural characters, and rDNA ITS sequence analysis.

关 键 词：米曲霉 黄曲霉 繁茂膜海绵 氧爆发 诱导抗性 

分 类 号：Q939.11[生物学—微生物学]