灵菌红素分批发酵过程的溶氧和补料调控  

Regulation of DO and Feeding in Process of Prodigiosin Batch Fermentation

作　　者：陈艳[1] 谌颉[1] 张佑红[1] 朱雄伟[1] 钟季良 荣广建 

机构地区：[1]武汉工程大学化工与制药学院绿色化工过程省部共建教育部重点实验室,湖北武汉430074

出　　处：《化学与生物工程》2015年第8期56-59,共4页Chemistry & Bioengineering

摘　　要：以粘质沙雷氏菌ZSG为受试菌株,对灵菌红素分批发酵过程的溶氧和补料方式进行调控。分别以恒转速（200r·min-1）与恒溶氧（10%、30%、50%）方式控制灵菌红素分批发酵过程,结果表明,与恒转速相比,恒溶氧控制方式能明显提高菌体干重,且在一定浓度范围内促进了灵菌红素的合成,在溶氧浓度为30%时,灵菌红素产量可提高69.0%。将溶氧浓度恒定在30%、维持最适pH值为6.7～7.8,通过流加补料,可使灵菌红素产量从补料前的79.01mg·L-1提高到115.98mg·L-1,提高了46.8%。Dissolved oxygen（DO） concentration and feeding modes for prodigiosin（PG） batch fermentation were regulated using Serratia marcescens ZSG as testing strain. The process of PG batch fermentation under constant agitation rate of 200 r · min-1 and constant DO concentrations of 10% ,30% and 50% were investiga- ted. Compared with constant agitation rate, constant DO concentrations significantly increased dry cell weight （DCW） and obviously promoted PG production within a reasonable range, PG production was improved by 69.0% under the constant DO concentration of 30%. Then the feeding mode of batch fermentation process was further studied under the constant DO concentration of 30%, optimum pH value of 6.7～7.8 and with flow feeding,the PG production was increased by 46.8% from 79.01 mg· L-1 to 115.98 mg · L-1.

关 键 词：灵菌红素 粘质沙雷氏菌 溶氧 补料 分批发酵 调控 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] Q939.97[生物学—微生物学]