雀嘴茶咖啡酰熊果苷水提工艺的优化  

Optimization of the water extraction process of 6'- O- caffeoylarbutin from Vaccinium dunalianum

作　　者：李娜[1] 但汉龙[1] 刘云[1] 罗旭璐 贺鹏[1] 阚欢[1] 赵平[1] 

机构地区：[1]西南林业大学西南山地森林资源保育与利用省部共建教育部重点实验室,云南昆明650224

出　　处：《食品工业科技》2015年第17期182-186,191,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(21462040;31460076);西南林业大学科技创新基金项目(C1463)

摘　　要：采用水浴法提取雀嘴茶中的6'-O-咖啡酰熊果苷,研究水浴温度、液料比、提取次数、水浴时间对6'-O-咖啡酰熊果苷得率的影响。在单因素实验基础上,利用中心组合设计原理和响应面分析法对6'-O-咖啡酰熊果苷的提取工艺条件进行了优化。结果表明,水浴提取6'-O-咖啡酰熊果苷的最佳工艺条件为:水浴温度72℃、液料比12∶1(m L/g)、提取次数4次和水浴时间60 min。在此优化条件下,6'-O-咖啡酰熊果苷平均得率为19.63%,与理论预测值较为接近,说明采用中心组合响应面法分析优化雀嘴茶6'-O-咖啡酰熊果苷水浴提取工艺的方法可行。Response surface method was applied to optimize the 6＇- O- caffeoylarbutin extraction conditions of Vaccinium dunalianum buds.Water bath was used to extract 6＇- O- caffeoylarbutin from V.dunalianum buds in the present work. Four extraction parameters including water temperature,liquid- material ratio,extraction times and water- bath time were discussed. Based on the single factor investigations,the optimal 6＇- O- caffeoylarbutin extraction conditions were obtained by central- composite design and response surface methodology.As a result,the optimum water bath extraction conditions of 6＇-O-caffeoylarbutin were： 72 ℃ of water temperature,12∶1（ m L / g）of liquid- material ratio,4 times of extraction number and 60 min of water- bath time. Under these conditions,the yield of 6＇- O- caffeoylarbutin could reach to 19.63% that was in consistency with the theoretic value.It was feasible to use central- composite response surface method to optimize the water bath extraction of 6＇- O- caffeoylarbutin from the buds of V.dunalianum.

关 键 词：雀嘴茶 6'-O-咖啡酰熊果苷 水浴提取 响应面法 

分 类 号：TS201.1[轻工技术与工程—食品科学]