动物双歧杆菌A6缓解小鼠便秘作用的研究  

Defecating Function of Bifidobacterium. animalis subsp. Lactis A6 Strain in Constipation Mice

作　　者：刘治麟 白海林 张建铭 刘力[4] 桑跃 武永超[4] 赵亮[4] 

机构地区：[1]北京和益源生物技术有限公司,北京100089 [2]海城市佳鑫牧业发展有限公司,海城114200 [3]江西鸿燕生物技术有限公司,南昌320001 [4]中国农业大学食品科学与营养工程学院北京市高等学校畜产品工程研究中心食品质量与安全北京实验室功能乳品教育部北京市共建重点实验室,北京100083

出　　处：《中国奶牛》2015年第15期32-35,共4页China Dairy Cattle

基　　金：中央在京高校重大成果转化项目"益生菌生产关键技术科技成果转化";南方大城市奶牛健康养殖生产技术集成及产业化示范(2012BAD12B08)

摘　　要：动物双歧杆菌A6是一株具有较强耐受性能的双歧杆菌菌株。本研究利用动物实验评价该菌株的缓解便秘作用。将Balb/c雄性小鼠随机分为空白组、模型组、A6不同剂量组。模型组、A6组使用复方地芬诺酯建立小鼠便秘模型,之后三组模型鼠分别给予低、中、高剂量(1×106、1×108、1×1010CFU/m L)的A6活菌液0.1m L/(kg·d),空白组和模型组灌胃0.1m L/(kg·d)(以体质量计)质量分数10%的脱脂乳。利用墨汁推进方法评价排便情况。动物双歧杆菌A6给药7d后,便秘小鼠首次排便时间缩短,排便粒数和质量明显增加,粪便水分含量提高;连续15d灌胃动物双歧杆菌A6,便秘小鼠小肠墨汁推进率也得到显著增加。结果表明,动物双歧杆菌A6具有良好的润肠通便功能。Bifidobacterium. animalis subsp. Lactis A6 is a probiotic strain which has strong probiotic activity. To evaluate the defecating function of this strain, male Balb/c mice were randomly divided into control group, model control group, positive control group and 3 dosages group. Control group as well as model control group were orally administered with 10% skim milk liquid 0.1mL/（kg·d） dose once a day. Select B. anirnalis subsp. And mice of A6 dosage group were administered with different concentrations （1×10^6. 1 ×10^8. 1×10^10 CFU/mL） at 0.1 mL/ （kg·d）. Mice constipation model was established by using compound diphenoxylate. The mice administrated with B. animalis subsp. Lactis A6 for 7 days had the shorter time of defecation of the first black feces, and the number and weight of mice feces within 5 hours were increased significantly while water content in the feces were also increased; when the mice were administered with B. animalis subsp. Lactis A6 stain for 15 days, the ink propelling rates were raised. The results verify that B. animalis subsp. Lactis A6 strain has the potential favorable defecation function in constipation mice.

关 键 词：动物双歧杆菌 通便 小鼠 便秘 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]