生物硫铁生成菌的选育及处理重金属效果研究  

Isolation of a biological iron sulfide composites-producing strain and its application in treatment of heavy metal wastewater

作　　者：杨阳[1,2] 谢翼飞[2] 朱德文[3] 谷晋川[1] 冯栩[1] 魏春梅[1] 

机构地区：[1]西华大学食品与生物工程学院,四川成都610039 [2]中国科学院成都生物研究所,四川成都610041 [3]四川大学轻纺与食品学院,四川成都610041

出　　处：《生物技术》2015年第4期391-396,共6页Biotechnology

基　　金：国家自然科学基金项目("生物硫铁复合材料处理重金属污染事故的应用基础研究";No.51378013);四川省教育厅一般项目("污酸中汞的赋存状态及脱除机理研究";No.13ZB0021)资助

摘　　要：[目的]分离高产生物硫铁生成菌,初步鉴定并研究其生成限制因素及处理重金属效果。[方法]利用分离驯化出的1株高产生物硫铁复合材料(生物硫铁)的硫酸盐还原菌(srb1),考察硫酸亚铁浓度、有机物浓度以及搅拌速度对其生成生物硫铁的影响。[结果]有机物和Fe SO4·7H2O的浓度是制约生物硫铁生成的关键因素,生物硫铁处理重金属Cr6+、Pb2+、Cd2+、Cu2+效果显著,去除率达90%以上,尤其是处理重金属Cu2+废水最佳,去除率达99%以上。[结论]筛选到生物硫铁生成菌srb1,初步鉴定为梭状芽胞杆菌属Clostridium mesophilum。确定了生物硫铁生成的最佳培养条件为乳酸钠15 m L/L+酵母膏8 g/L+Fe SO4·7H2O 15 g/L。[ Objective ] Isolate a strain that high -yield biological iron sulfide composites preliminarily identify it and study its generation limiting factor and the treatment effect on heavy metal wastewater. [ Methods] A strain of sulfate -reducing bacteria with high yield of biological iron sulfide composites （BISC） was separated and acclimated. The effect of concentration of ferrous sulfate and organics and stirring speed on the generation of biological iron sulfide composites was studied. [ Results ] The concentration of organic and FeSO4＋ 7H2O was the limiting factor in BISC generation. BISC had remarkable treatment effect on Cr6 ＋ , Pb2＋ , Cd2 ＋ , Cu2 ＋ heavy metal wastewater, removal efficiency reached 90%, especially Cn2 ~ wastewater, the removal effi- ciency reached 99%. [ Conclusion] A strain srbl that high -yield BISC was isolated and identified as Clostridium mesophilum. The optimal culture conditions were determined as sodium lactate 15 mL/L,yeast extract 8 g/L and FeSO4·7H2O 15 g/L.

关 键 词：硫酸盐还原菌 生物硫铁 重金属废水 处理效果 

分 类 号：X172[环境科学与工程—环境科学]