勃氏甜龙竹水对高血脂大鼠降血脂作用及脂代谢相关基因表达的影响  

Effects of Dendrocalamus brandisii Juice on Reducing Blood Lipid and Genes Expression Profiles Related to Lipid Metabolism in Hyperlipoidemic Model Rats

作　　者：钟禄彪 徐永敏[1] 邓杰文 冯悦[1] 王成[1] 李峥[3] 夏雪山[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,昆明650500 [2]普洱市滇润农业科技有限公司,普洱666500 [3]云南省第一人民医院检验科,昆明650500

出　　处：《天然产物研究与开发》2015年第8期1396-1401,1339,共7页Natural Product Research and Development

基　　金：国家自然科学基金(81260248;81360247);国家科技支撑计划(2014BAI01B01)

摘　　要：为了开发利用资源丰富的勃氏甜龙竹水,对勃氏甜龙竹水的常规成分进行分析,并探讨其对高血脂症大鼠降血脂作用及其可能的分子机制。经对勃氏甜龙竹水进行总黄酮含量、总酚含量、总糖含量、总蛋白质含量及氨基酸含量测定,确定其总糖量为25.3%,蛋白质含量为11.4%,总酚含量为0.35%,总黄酮含量为0.085%,总氨基酸含量为8.114%,其中含有7种必需氨基酸。采用高脂饲料饲喂法建立高血脂大鼠模型,从勃氏甜龙竹下部采取竹水饲喂高血脂大鼠,在不同时间进行大鼠尾静脉采血,测定各组实验动物的血清血脂水平,并测定大鼠肝脏中与血脂代谢相关的基因mRNA相对表达量。结果发现,勃氏甜龙竹水能有效降低高血脂症大鼠的血清总胆固醇(TC)、低密度脂蛋白(LDL-C)的含量,同时能提高大鼠血清高密度脂蛋白(HDL-C)含量和肝脏LXRα、ABCA1基因mRNA的表达量。勃氏甜龙竹水能够在一定程度上降低血脂水平,预防高脂血症的发生,该作用可能与上调LXRα、ABCA1基因表达,进而促进胆固醇逆转运有关。To develop and utilize the juice of Dendrocalamus brandisii, its composition and molecular mechanism of hypolipidemic effect were investigated.With the biomedical analysis,it was determined that its components include total sugar,flavonoids,phenolics,protein and amino acids with the ratios of 25.3%, 0.085%, 0.35%,11.4% and 8.114%,respectively.Furthermore,amino acids contained seven kinds of essential amino acids.Hyperlipidemia rat models were established by high-fat diet feeding,following with the feeding of D.brandisii to investigate its hypolipidemic effect.The blood was collected from coccygeal vein of rats at different times;the serum lipid levels were further detected.The expression level of LXRα and ABCA1 gene in the liver were quantitatively determined.The results showed that the serum levels of TC and LDL-C were decreased in rats with juice feeding,but the HDL-C level and the gene expression of LXRα and ABCA1 in liver tissue were increased.In conclusion,D.brandisii juice might significantly reduce the blood lipid level and prevent hyperlipidemia may through up-regulating the expression of ABCA1 and LXRα and further promoting the transportation of cholesterol.

关 键 词：勃氏甜龙竹 竹水 降血脂 分子机制 

分 类 号：R151.3[医药卫生—营养与食品卫生学]