无溶剂体系中固定化脂肪酶催化合成香茅醇酯  

Synthesis of Citronellyl Esters Catalyzed by Immobilized Lipase in Solvent-Free System

作　　者：于敏[1] 周华[1] 任立伟[1] 蒋振华[1] 韦萍[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800

出　　处：《化学反应工程与工艺》2015年第4期373-378,共6页Chemical Reaction Engineering and Technology

基　　金：国家重点基础研究计划(973)项目(2009CB724706)

摘　　要：在无溶剂体系中以大孔树脂NKA吸附固定的褶皱假丝酵母脂肪酶(Candida rugosalipase)为催化剂,以脂肪酸为酰基供体,酶法合成香茅醇脂肪酸酯。考察了褶皱假丝酵母脂肪酶对酰基供体的选择性,以及反应温度、摇床转速、酶用量、底物比例及微量水添加等对酯化反应的影响,建立了无溶剂中香茅醇酯的酶法合成工艺。研究表明,在酸醇物质的量比为1,摇床转速为200 r/min,固定化酶用量为1250 U/L,反应温度为50℃,微量水添加量为4μL的条件下,反应10h,月桂酸香茅酯及油酸香茅酯的转化率分别可达95.6%和87.6%,明显高于在有机溶剂体系中的酯化率。The enzymatic synthesis of citronellyl esters by esterification of citronellol and fatty acids in a solvent-free system was discussed using Candida rugosa lipase (CRL) absorbed on macroporous resin NKA as catalyst. The conditions of enzymatic synthesis of citronellyl esters was optimized, including the selectivity of acyl donor, reaction temperature, enzyme dosage, molar ratio of substrate and micro-water addition. The results showed that under the conditions of molar ratio of acid to alcohol 1, agitation speed 200 r/min, reaction temperature 50℃, the concentration of immobilized lipase 1 250 U/L, the addition of water 4μL, citronellyl laurate and citronellyl oleate were achieved with esterification rate of 95.6% and 87.6%, respectively, after 10 h of reaction, which was more superior than that in organic solvent system.

关 键 词：香茅醇酯 脂肪酶 固定化 无溶剂 酶催化 

分 类 号：Q814.9[生物学—生物工程]