大蒜不同极性萃取物的体外抗氧化活性  

Studies on the antioxidatant activities of different fractions of garlic extract

作　　者：邢利沙 陈海霞[1] 王佳[1] 王艳伟[1] 

机构地区：[1]天津大学药物科学与技术学院,天津市现代药物传递及功能高效化重点实验室,天津300072

出　　处：《食品与发酵工业》2015年第4期219-222,共4页Food and Fermentation Industries

基　　金：国家自然科学基金(No:31371879)资助

摘　　要：为了研究大蒜不同极性萃取物的抗氧化活性,确定大蒜的活性部位,促进大蒜资源在天然抗氧化剂和药品等领域的应用,文中采用体积分数80%乙醇回流提取、梯度萃取得到石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水萃取物4个不同极性部位。以清除二苯代苦味酰基(DPPH)自由基、还原力和对肝脂质过氧化抑制力为评价指标研究其体外抗氧化活性。结果表明:大蒜不同极性萃取物均有一定的抗氧化活性,其抗氧化能力与不同极性萃取物的质量浓度呈现出良好的线性依赖关系,抗氧化效果因反应体系不同而有所不同。正丁醇萃取物在3个体外抗氧化活性模型中均表现出较好的抗氧化活性,其清除DPPH自由基和肝脂质过氧化抑制活性的半数抑制浓度分别为0.46 mg/m L和0.36 mg/m L。大蒜正丁醇萃取物得率较高(7.86%),抗氧化活性较好,可以作为新型有效的天然抗氧化物质进行开发利用。To investigate the in vitro antioxidant activities of different fractions of garlic extract and to find out the active fraction of garlic for exploitation in natural antioxidant and drug applications,the extracts were prepared by80% ethanol circumfluence extraction. Four fractions were obtained by extraction with different polar solvents including petroleum ether,ethyl acetate,normalbutyl alcohol and water. The antioxidant activities of the fractions were compared and assessed by three in vitro antioxidant assays including 1-diphenyl-2-picrylhydrazyl（ DPPH） radical scavenging,ferric reducing power and inhibition on liver lipid peroxidation. Results showed that all the fractions had antioxidant activity and the antioxidant capacity was different. There was a good linear dependence between the capacity and the concentration. N-butyl alcohol fraction showed better antioxidant activity. The half inhibition concentration of DPPH scavenging rate was 0. 46 mg / m L and half inhibition concentration of liver lipid peroxidation was 0. 36 mg /m L. N-butanol extract had higher yields（ 7. 86%） and good antioxidant activity,therefore could be used as a good source of natural antioxidants.

关 键 词：大蒜 DPPH 还原力 肝脂质过氧化抑制 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]