固定化乳酸菌制备生物传感器测定抗生素  

Biosensor Preparation for Detecting Antibiotics by Immobilized Lactobacilli

作　　者：乔立新[1] 吴苏生 庞广昌[1] 

机构地区：[1]生物技术与食品科学学院天津商业大学天津市食品与生物技术重点实验室,天津300134

出　　处：《食品科学》2015年第16期261-265,共5页Food Science

基　　金：国家自然科学基金面上项目(31371773)

摘　　要：用海藻酸钠-淀粉凝胶作固定剂,将乳酸菌固定到两片核微孔膜中间制成"三明治"式传感膜,然后将其固定到玻碳电极上制成生物传感电极。通过电化学工作站检测3种抗生素分别在不同质量浓度条件下的响应电流,结果表明:该传感器固定瑞士乳杆菌的最适量为0.05 g,此时对青霉素、链霉素、四环素的最低检测限分别为1×10-10、1×10-9、1×10-9 g/m L,检出时间为4 min,明显优于国内外对抗生素残留量的要求。而且经测定表明该电极在37℃的MRS培养基中至少可以稳定保存7 d,低温保藏应该可以保存更长时间,说明电极性能比较稳定。总之,固定化乳酸菌制备的生物传感器提供了一种新的定量测定抗生素的方法,其检测灵敏度高、成本低、简单、快速,不仅适用于乳制品中抗生素残留的定量化快速检测,而且可以实现对多种类型的抗生素进行检测。Using sodium alginate-starch gel as a fixing agent, lactobacilli were immobilized between two nuclear microporous membranes to make a sandwich-type sensing membrane, which was then fixed to a glassy carbon electrode to make a biosensor electrode. Based on the current responses of three antibiotics at different concentrations tested by electrochemical workstation, the calculation results showed that the most appropriate quantity of lactobacilli fixed by the sensor was 0.05 g, and now the limits of detection concentration（LDC） of the biosensor for penicillin, streptomycin and tetracycline were 1 × 10-10, 1 × 10-9 and 1 × 10-9 g/m L, respectively. The detection time was 4 min, which was obviously better than the requirement for the analysis of residual antibiotics at home and abroad. The electrode in 37 ℃ MRS medium could remain stable for 7 days at least, and the low temperature preservation time was extended, suggesting that the electrode performance was stable. In total, the microbial sensor by immobilized lactobacilli provides a new method for quantitative determination of antibiotics with high sensitivity, low cost, simple operation and fast detection. Therefore, it is not only applicable to the quantification and rapid detection of residual antibiotics in milk products, but also can detect a variety of types of antibiotics.

关 键 词：瑞士乳杆菌 生物传感器 青霉素 链霉素 四环素 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]