甘油生产1,3-丙二醇发酵工艺优化研究  

Optimization of Fermentation Process for Producing 1,3-PDO From Glycerol

作　　者：姚新武[1] 张霖[1] 樊亚超[1] 

机构地区：[1]中国石化抚顺石油化工研究院,辽宁抚顺113001

出　　处：《当代化工》2015年第8期1813-1815,共3页Contemporary Chemical Industry

基　　金：中国石油化工集团公司资助项目;项目号SH1112

摘　　要：1,3-丙二醇是合成聚对苯二甲酸丙二醇酯的基础原料,利用甘油进行微生物发酵生产1,3-丙二醇的生物炼制技术具有广阔的应用前景。以克雷伯氏肺炎杆菌为出发菌种,对菌种保藏方式、发酵体系环境、氮气通气比、p H中和剂以及甘油品质等发酵工艺进行了优化研究。实验结果表明,在较优的工艺条件下,1,3-丙二醇产量可达103.38 g/L。1,3-PDO is the basic raw material to produce polytrimethylene terephthalate. Biorefinery technology of producing 1,3-PDO from glycerol with ferment has broad application prospect. In this paper, K. pneumoniae was chosen as the starting strain. Fermentation process parameters including strain preservation methods, fermentation system environment, nitrogen ventilation ratio, pH neutralizing agent and glycerol quality were optimized. The experimental results show that the yield of 1,3-PDO can reach to 103.38 g/L under optimum process conditions.

关 键 词：克雷伯氏肺炎杆菌 1 3-丙二醇 发酵工艺 优化 

分 类 号：TQ923[轻工技术与工程—发酵工程]