改良自酸蚀粘接剂对龋影响牙本质粘接性能的影响  

Effect of epigallocatechin-3-gallate to self-ectching adhisive to the bonding strengh of cariesaffected dentin

作　　者：李小龙[1] 覃峰[1] 钱宇[1] 尹映竹 罗涛[2] 黄雪清[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055 [2]广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室,510140

出　　处：《中华口腔医学研究杂志（电子版）》2015年第4期27-30,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：教育部博士点基金(新教师类;20130171120121);国家自然科学基金(青年科学基金项目;81300902);广东省科技计划(2011B031300018);中山大学青年教师培育计划(12ykpy62)

摘　　要：目的：探讨表没食子儿茶素没食子酸酯（EGCG）改良后自酸蚀粘接剂可乐丽Clearfil SE Bond对龋影响牙本质即刻粘接性能影响。方法选取中龋离体牙20颗,在龋指示剂下去除龋感染牙本质,保留龋影响牙本质。将EGCG配制成100、200、300μg/ml Clearfil SE Bond粘接剂溶液及水溶液,以不添加EGCG粘接剂及水溶液作为对照组。微拉伸试件分组按照Clearfil SE Bond说明书指导进行粘接,上方堆塑5-6 mm Z350树脂。使用慢速线性切割机沿牙齿长轴进行切割,形成约1 mm ×1 mm试件,进行微拉伸实验。显微硬度试件分组浸泡于EGCG水溶液中24 h后测试显微硬度。使用SPSS 22.0统计学软件,在One-Way ANOVA模式下进行统计学分析。结果 EGCG处理后龋影响牙本质的粘接强度及显微硬度均明显提高,差异有统计学意义（F粘接强度=24.554,P粘接强度﹤0.05；F 显微硬度=20.418,P显微硬度﹤0.05）。结论 EGCG可增强龋影响牙本质的即刻粘接强度及显微硬度。Objective To explore the effect of the modified Epigallocatechin-3-Gallate （EGCG） on self-etch Clearfil SE Bond bonding strength to caries-affected dentin. Methods Twenty extracted human teeth with medial coronal carious lesions extending into middle dentin were used. The softened carious dentin was removed by Caries Marker. EGCG was incorporated at a ratio of 100, 200, and 300 μg/ml into water solution and a dental adhesive, Clear SE Bond. Caries-affected dentin was treated with EGCG water solution for 24 hours before micro-hardness test. And Microtensile bond strength （μTBS） test was used to evaluate the bonding strength to caries-affected dentin treated by Clearfil SE Bond with different EGCG concentration. Statistics analysis software SPSS 22.0 was used to evaluate the difference by One-Way ANOVA. Result Micro-hardness and bonding strength of caries-affected dentin are both significantly increased when treated with EGCG （Fbonding strength=24.554, Pbonding strength﹤0.05;Fmicro-hardnes=20.418, Pmicro-hardnes﹤0.05）. Conclusion EGCG can significantly increase the micro-hardness and bonding strength of caries-affected dentin.

关 键 词：表没食子儿茶素没食子酸酯 龋影响牙本质 粘接强度 显微硬度 

分 类 号：R781.1[医药卫生—口腔医学]