肺腺癌组织中基质窖蛋白-1和LDH-B、PKM2的表达及其意义  

Expression of stromal caveolin-1,LDH-B and PKM2 in pulmonary adenocarcinoma

作　　者：陈福春 潘琦 傅品 陆富年[2] 陈洪雷 

机构地区：[1]温岭市中医院胸外科,317500 [2]武汉大学基础医学院病理学教研室

出　　处：《浙江医学》2015年第16期1363-1366,共4页Zhejiang Medical Journal

基　　金：国家自然科学基金资助项目(编号:30500226)

摘　　要：目的探讨基质窖蛋白1(Cav-1)、乳酸脱氢酶B(LDH-B)和丙酮酸激酶2(PKM2)在人肺腺癌(PAC)组织中的表达并分析3者的相关性及其临床意义。方法采用量子点免疫荧光组织化学(QDs-IHC)技术检测68例PAC组织和16例非癌性肺组织中基质Cav-1、LDH-B和PKM2的表达情况。结果基质Cav-1在PAC组织的阳性率为58.8%,与非癌变肺组织的阳性率100%相比,差异有统计学意义(P<0.05)。肺腺癌组织中LDH-B和PKM2的阳性率分别为76.5%、70.6%,均高于非癌性肺组织(均P<0.05)。PAC中、低分化组PKM2的阳性率均高于高分化组(均P<0.05)。结论基质Cav-1缺失,LDH-B、PKM2的高表达可能促进PAC的恶性进展。Objective To investigate the expression of stromal caveolin-1 （Cav-1）, L-lactate dehydrogenase B （LDH-B） and pyruvate kinase M2（PKM2） in pulmonary adenocarcinoma（PAC） tissues. Methods The expression of stromal Cav-1, LDH-B and PKM2 proteins in 68 specimens of PAC and 16 specimens of noncancerous lung tissue with quantum dots immunofluorescent histochemistry（QDs-IHC）, Results The positive rates of stromal Cav-1 in PAC tissues and non-cancerous lung tissues were 58.8% and 100.0%（P〈0.05）. The positive rates of LDH-B and PKM2 expression in PAC tissues were 76.5% and 70.6%, respectively, which were higher than those in noncancerous lung tissues（P〈0.05）. The expression of PKM2 in mid- dle/low-differentiated PAC was higher than that in well-differentiated PAC（P〈0.05）. Conclusion The results indicated that stro- real Cav-1 is down-regulated and LDH-B and PKM2 are up-regulated in pulmonary adenocarcinoma.

关 键 词：肺腺癌 窖蛋白1 丙酮酸激酶2 肿瘤能量代谢 CAVEOLIN-1 PYRUVATE kinase M2 

分 类 号：R734.2[医药卫生—肿瘤]