异养小球藻油脂正己烷提取工艺优化  

Optimization of oil extraction from heterotrophic Chlorella by hexane

作　　者：毕生雷 张成明[2,3] 卢悦[4] 郑世文[1] 金洪波[1] 吴娟[1] 鲁龙[1] 

机构地区：[1]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,河南南阳473000 [2]清华大学核能与新能源技术研究院,北京100084 [3]北京市生物燃料工程技术研究中心,北京100084 [4]清华大学生命科学院,北京100084

出　　处：《中国油脂》2015年第9期18-21,共4页China Oils and Fats

基　　金：国家科技支撑计划(2011BAD14B05)

摘　　要：为确定最佳异养小球藻油脂正己烷提取工艺,对提取工艺中的关键参数如藻细胞溶液质量浓度、破碎压力、破碎次数、萃取时间、萃取温度、萃取剂正己烷添加量对油脂得率的影响进行了研究。结果表明,最佳油脂提取工艺条件为藻细胞溶液质量浓度110 g/L、破碎压力100 MPa、破碎次数4次、萃取时间5 h、萃取温度40℃、正己烷添加量9 m L/g(以藻粉干重计),在该条件下萃取5次,油脂得率最高,可以达到55%左右。The effects of key parameters such as mass concentration of algae cell solution, crushing pres- sure, crushing times, extraction time, extraction temperature and dosage of extractant （hexane） on the oil yield were studied so as to obtain the optimal oil extraction process from heterotrophic Chlorella by hex- ane. The results showed that the optimal oil extraction process was obtained as follows ： mass concentration of alage cell solution 110 g/L, crushing pressure 100 MPa, crushing times 4 times, extraction time 5 h, ex- traction temperature 40 ~C, dosage of hexane 9 mL/g（based on the mass of dry algae powder）. Under these conditions,the oil yield could reach the highest about 55% after extraction for 5 times.

关 键 词：异养小球藻 细胞破碎 萃取 油脂得率 

分 类 号：TQ644[化学工程—精细化工]