高效液相色谱法测定玉米中的游离酚和结合酚  

Determination of Soluble Phenolics and Bound Phenolics in Corn by High Performance Liquid Chromatography

作　　者：李艳[1,2] 刘梅森 万红霞[2] 孙海燕[2] 从彦丽[2] 许柏球[2] 刘冬[2] 

机构地区：[1]华南理工大学轻工与食品学院,广州510640 [2]深圳职业技术学院深圳市发酵精制检测系统重点实验室,深圳518055 [3]深圳市味奇生物科技有限公司,深圳518109

出　　处：《中国粮油学报》2015年第9期108-111,共4页Journal of the Chinese Cereals and Oils Association

基　　金：广东省国际合作项目(2012B050600031);省部产学研结合项目(2012B091100411);深圳职业技术学院青年创新项目(601422K27012)

摘　　要：建立了高效液相色谱法同时测定玉米中游离酚和结合酚的检测方法,包括原儿茶酸、龙胆酸、对羟基苯甲酸、绿原酸、香草酸、咖啡酸、丁香酸、表儿茶素、香豆酸、阿魏酸、芥子酸、芦丁、橙皮素、槲皮素。有机溶剂提取玉米中的游离酚,碱法提取玉米中的结合酚,以ZORBAX Eclipse Plus Phenyl-Hexyl（4.6 mm×250mm,5μm）为分析柱,乙腈（A）和含0.4%乙酸的超纯水（B）为流动相梯度洗脱,采用二极管阵列检测器280 nm波长检测。14种酚类化合物在一定浓度范围内与其峰面积呈良好的线性关系,相关系数为0.999 4-0.999 9,加标回收率为90%-108%,相对标准偏差为0.03%-3.59%。试验结果表明,该方法能用来同时测定玉米中的游离酚和结合酚。An effective high performance liquid chromatographic （HPLC） method had been developed for the determination of soluble phenolics and bound phenolics, including protocatechuic acid. 2.5 - Dihydroxybenzoic acid, 4 -Hydroxybenzoic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, Epi＇catechin. coumalic acid, ferulic acid, sinapic acid, rutin, hesperetin and quercetin. Soluble phenolics were extracted with organic solvent; bound phenolics were extracted by alkaline. The separation and quantification of soluble phenolics and bound phenolies were achieved on ZORBAX Eclipse Plus Phenyl - Hexyl （4.6 mm × 250 mm, 5 μm） with mobile phase of acetonitrile （A） and 0.4% acetic acid （B） with diode array detector （DAD） at 280 nm. The results indicated that the calibration curves of fourteen phenolics had expressed a satisfied linear relationships between peak areas and concentrations, with their correlation coefficients of 0. 999 4 - 0. 999 9, The average recoveries of fourteen phenolics were in the range of 90%-108% with relative standard deviations of 0.03%-3.59% , The method shall be suitable for determination of soluble phenolics and bound phenolics in corn.

关 键 词：玉米 游离酚 结合酚 高效液相色谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]