不同产地杜仲叶中5种主要有效成分的含量比较  

Comparison of Contents of Five Effective Components in Eucommiae Folium from Different Areas

作　　者：刘荣华[1] 唐芳瑞[1] 陈兰英[1] 邵峰[1] 李于益 张忠立[1] 黄慧莲[1] 

机构地区：[1]江西中医药大学,南昌330004

出　　处：《中国实验方剂学杂志》2015年第18期31-34,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81160508)

摘　　要：目的：比较17个不同产地杜仲叶中5种主要有效成分的含量差异。方法：利用高效液相色谱法（HPLC）测定桃叶珊瑚苷、京尼平苷酸、绿原酸、儿茶素、芦丁的含量,流动相乙腈（A）-0.1%磷酸水溶液（B）梯度洗脱（0-45 min,5%-15%A;45-85 min,15%-24%A）,检测波长208 nm。考察不同产地杜仲叶中5种有效成分的含量差异。结果：不同产地杜仲叶中5种主要有效成分总量及各有效成分含量之间均存在显著差异,其中采自陕西省黄陵县的杜仲叶中5种有效成分总质量分数高达14.15%,而采自四川省渠县的杜仲叶仅1.37%,两者相差〉10倍。结论：杜仲叶中主要有效成分含量存在一定的地域性差异,其中陕西、河南等地样品中含量明显高于西南地区（如四川、重庆、贵州等）。杜仲叶应尽可能采取低温快速干燥。Objective： To compare contents of five main effective components （aucubin, geniposidic acid, chlorogenic acid, catechin and rutin） in Eucommiae Folium from 17 different areas. Method： HPLC was employed to determine contents of five main effective components with mobile phase of acetonitrile-0. 1% phosphoric acid for gradient elution and detection wavelength at 208 nm. Contents of five main effective components in Eucommiae Folium from 17 different areas were investigated. Result： There were significant differences between contents and total content of these five main effective components in Eucommiae Folium from different areas. The highest total content of five effective components was 14.15% which was from Huangling county, Shaanxi province; samples with the lowest total content （ 1.37% ） of them was from Quxian county, Sichuan province; difference of these two values was more than 10-fold. Conclusion： There are some regional differences of contents of five main effective components in Eucommiae Folium. The total content of main effective components in samples from Shaanxi and Henan province is more than from southwest area of our country, such as Sichuan, Guizhou province and Chongqing city. Eucommiae Folium should be possible to take low temperature rapid drying.

关 键 词：杜仲叶 桃叶珊瑚苷 京尼平苷酸 绿原酸 儿茶素 芦丁 

分 类 号：R284.1[医药卫生—中药学] R282.4[医药卫生—中医学]